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Literature summary for 1.4.9.2 extracted from

  • Hothi, P.; Abu Khadra, K.; Combe, J.P.; Leys, D.; Scrutton, N.S.
    Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis. Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitrificans. [Erratum to document cited in CA144:14530] (2006), FEBS J., 273, 430.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information analysis of limiting rate constants of reaction with benzylamine and deuterated benzylamine at different pH values Alcaligenes faecalis
6.7
-
benzylamine pH 7.5, 25°C, native enzyme Alcaligenes faecalis
9.9
-
benzylamine pH 7.5, 25°C, recombinant enzyme Alcaligenes faecalis

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
expression in Paracoccus denitrificans
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, expression in Paracoccus denitrificans Alcaligenes faecalis

Reaction

Reaction Comment Organism Reaction ID
ArCH2NH2 + H2O + 2 azurin = ArCHO + NH3 + 2 reduced azurin breakage of the substrate C-H bond occurs via quantum mechanical tunneling. In steady-state reactions with benzylamine, breakage of C-H bond is only partially rate-limiting Alcaligenes faecalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30.8
-
pH 7.5, 25°C Alcaligenes faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzylamine + H2O + 2,6-dichlorophenol analysis of limiting rate constants Alcaligenes faecalis ? + NH3 + reduced 2,6-dichlorophenol indophenol
-
?
phenazine ethosulfate + H2O + 2,6-dichlorophenol indophenol
-
Alcaligenes faecalis ? + NH3 + reduced 2,6-dichlorophenol indophenol
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.02
-
benzylamine pH 7.5, 25°C, recombinant enzyme Alcaligenes faecalis
1.14
-
benzylamine pH 7.5, 25°C, native enzyme Alcaligenes faecalis