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Literature summary for 1.4.9.2 extracted from

  • Hyun, Y.L.; Davidson, V.L.
    Electron transfer reactions between aromatic amine dehydrogenase and azurin (1995), Biochemistry, 34, 12249-12254.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.044
-
azurin in 0.25 M potassium phosphate buffer Alcaligenes faecalis
0.137
-
azurin in 0.01 M potassium phosphate buffer Alcaligenes faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
aromatic amines + acceptor + H2O Alcaligenes faecalis physiological acceptor: azurin, not amicyanin ? + reduced acceptor + NH3
-
?
aromatic amines + acceptor + H2O Alcaligenes faecalis IFO 14479 physiological acceptor: azurin, not amicyanin ? + reduced acceptor + NH3
-
?

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-
Alcaligenes faecalis IFO 14479
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ArCH2NH2 + H2O + 2 azurin
-
Alcaligenes faecalis ArCHO + NH3 + 2 reduced azurin
-
?
ArCH2NH2 + H2O + 2 azurin
-
Alcaligenes faecalis IFO 14479 ArCHO + NH3 + 2 reduced azurin
-
?
aromatic amines + acceptor + H2O physiological acceptor: azurin, not amicyanin Alcaligenes faecalis ? + reduced acceptor + NH3
-
?
aromatic amines + acceptor + H2O physiological acceptor: azurin, not amicyanin Alcaligenes faecalis IFO 14479 ? + reduced acceptor + NH3
-
?
tyramine + acceptor + H2O electron acceptor: azurin Alcaligenes faecalis 4-hydroxyphenylacetaldehyde + NH3 + reduced acceptor
-
?
tyramine + acceptor + H2O electron acceptor: azurin Alcaligenes faecalis IFO 14479 4-hydroxyphenylacetaldehyde + NH3 + reduced acceptor
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
64
-
azurin in 0.01 M potassium phosphate buffer Alcaligenes faecalis
91
-
azurin in 0.25 M potassium phosphate buffer Alcaligenes faecalis

Cofactor

Cofactor Comment Organism Structure
tryptophan tryptophylquinone
-
Alcaligenes faecalis