Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Synechocystis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | at high concentrations | Synechocystis sp. | |
6-diazo-5-oxo-norleucine | 0.05 mM, 99% inhibition | Synechocystis sp. | |
azaserine | 0.05 mM, 62% inhibition | Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
reduced ferredoxin | native enzyme | Synechocystis sp. | |
0.0045 | - |
reduced ferredoxin | recombinant enzyme | Synechocystis sp. | |
0.5 | - |
2-oxoglutarate | native enzyme | Synechocystis sp. | |
0.6 | - |
2-oxoglutarate | recombinant enzyme | Synechocystis sp. | |
2.2 | - |
L-glutamine | recombinant enzyme | Synechocystis sp. | |
2.5 | - |
L-glutamine | native enzyme | Synechocystis sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | enzyme contains a single [3Fe-4S] cluster | Synechocystis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
175000 | - |
recombinant enzyme, gel filtration | Synechocystis sp. |
178000 | - |
native enzyme, gel filtration | Synechocystis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + reduced ferredoxin | Synechocystis sp. | - |
L-glutamate + oxidized ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
PCC 6803, cyanobacterium | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, affinity chromatography on ferredoxin-Sepharose, native enzyme, partially purified | Synechocystis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
36.6 | - |
recombinant enzyme | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + reduced ferredoxin | - |
Synechocystis sp. | L-glutamate + oxidized ferredoxin | - |
? | |
L-glutamine + 2-oxoglutarate + reduced ferredoxin + H+ | - |
Synechocystis sp. | L-glutamate + oxidized ferredoxin | - |
r | |
L-glutamine + 2-oxoglutarate + reduced methylviologen | - |
Synechocystis sp. | L-glutamate + oxidized methylviologen | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
50% activity remains after 20 min, recombinant enzyme | Synechocystis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | one FMN per enzyme molecule | Synechocystis sp. |