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Literature summary for 1.4.7.1 extracted from

  • Navarro, F.; Martin-Figueroa, E.; Candau, P.; Florencio, F.J.
    Ferredoxin-dependent iron-sulfur flavoprotein glutamate synthase (GlsF) from the Cyanobacterium synechocystis sp. PCC 6803: expression and assembly in Escherichia coli (2000), Arch. Biochem. Biophys., 379, 267-276.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Synechocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate at high concentrations Synechocystis sp.
6-diazo-5-oxo-norleucine 0.05 mM, 99% inhibition Synechocystis sp.
azaserine 0.05 mM, 62% inhibition Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0035
-
reduced ferredoxin native enzyme Synechocystis sp.
0.0045
-
reduced ferredoxin recombinant enzyme Synechocystis sp.
0.5
-
2-oxoglutarate native enzyme Synechocystis sp.
0.6
-
2-oxoglutarate recombinant enzyme Synechocystis sp.
2.2
-
L-glutamine recombinant enzyme Synechocystis sp.
2.5
-
L-glutamine native enzyme Synechocystis sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Iron enzyme contains a single [3Fe-4S] cluster Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
175000
-
recombinant enzyme, gel filtration Synechocystis sp.
178000
-
native enzyme, gel filtration Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + 2-oxoglutarate + reduced ferredoxin Synechocystis sp.
-
L-glutamate + oxidized ferredoxin
-
?

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
PCC 6803, cyanobacterium
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, affinity chromatography on ferredoxin-Sepharose, native enzyme, partially purified Synechocystis sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36.6
-
recombinant enzyme Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + reduced ferredoxin
-
Synechocystis sp. L-glutamate + oxidized ferredoxin
-
?
L-glutamine + 2-oxoglutarate + reduced ferredoxin + H+
-
Synechocystis sp. L-glutamate + oxidized ferredoxin
-
r
L-glutamine + 2-oxoglutarate + reduced methylviologen
-
Synechocystis sp. L-glutamate + oxidized methylviologen
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
50% activity remains after 20 min, recombinant enzyme Synechocystis sp.

Cofactor

Cofactor Comment Organism Structure
FMN one FMN per enzyme molecule Synechocystis sp.