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Literature summary for 1.4.7.1 extracted from
- Glutamate synthase from rice leaves (1982), Plant Physiol., 69, 848-852.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azaserine | - |
Oryza sativa |  |
| Cd2+ | 5 mM, 69% inhibition | Oryza sativa | |
| Co2+ | 5 mM, 65% inhibition | Oryza sativa | |
| Mn2+ | 5 mM, 45% inhibition | Oryza sativa | |
| Ni2+ | 5 mM, 73% inhibition | Oryza sativa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0055 | - |
reduced ferredoxin | - |
Oryza sativa | |
| 0.33 | - |
2-oxoglutarate | - |
Oryza sativa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Oryza sativa | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 6% activation | Oryza sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 115000 | - |
2 * 115000, SDS-PAGE | Oryza sativa |
| 224000 | - |
gel filtration | Oryza sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 2-oxoglutarate + reduced ferredoxin | Oryza sativa | - |
L-glutamate + oxidized ferredoxin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
rice | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography on ferredoxin Sepharose | Oryza sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Oryza sativa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 35.88 | - |
- |
Oryza sativa |
| 35.9 | - |
- |
Oryza sativa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 5 mM dithiothreitol, 20 days, 54% loss of activity | Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 2-oxoglutarate + reduced ferredoxin | - |
Oryza sativa | L-glutamate + oxidized ferredoxin | - |
? | |
| L-glutamine + 2-oxoglutarate + reduced ferredoxin + H+ | - |
Oryza sativa | L-glutamate + oxidized ferredoxin | - |
r | |
| L-glutamine + 2-oxoglutarate + reduced methylviologen | artificial elctron donor | Oryza sativa | L-glutamate + oxidized methylviologen | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 115000, SDS-PAGE | Oryza sativa |
| monomer | SDS-PAGE | Oryza sativa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 137 | - |
L-glutamine | - |
Oryza sativa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
- |
Oryza sativa |
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Release 2023.1 (January 2023)
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