Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Helicobacter pylori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzoate | 0.1 mM, 98% inhibition | Helicobacter pylori | |
iodoacetamide | 0.1 mM, 66% inhibition | Helicobacter pylori | |
iodoacetic acid | 0.1 mM, 62% inhibition | Helicobacter pylori | |
additional information | EDTA, Mg2+ and Ca2+ have no effect on DadA activity | Helicobacter pylori | |
p-hydroxybenzoic acid | 0.1 mM, 79% inhibition | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0082 | - |
oxidized coenzyme Q1 | pH 8.0, 37°C | Helicobacter pylori | |
40.2 | - |
D-proline | pH 8.0, 37°C | Helicobacter pylori |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Mg2+ and Ca2+ have no effect on DadA activity | Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
x * 44000, SDS-PAGE | Helicobacter pylori |
46040 | - |
x * 46040, calculated from sequence | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | A3KEZ1 | NCTC 11637 | - |
Purification (Comment) | Organism |
---|---|
from Helicobacter pylori and recombinant Escherichia coli cells | Helicobacter pylori |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.22 | - |
- |
Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ala + oxidized coenzyme Q1 + H2O | specific activity is 37% of that with D-proline | Helicobacter pylori | pyruvate + NH3 + reduced coenzyme Q1 | - |
? | |
D-Phe + oxidized coenzyme Q1 + H2O | specific activity is 20% of that with D-proline | Helicobacter pylori | phenylpyruvate + NH3 + reduced coenzyme Q1 | - |
? | |
D-proline + oxidized coenzyme Q1 + H2O | - |
Helicobacter pylori | DELTA1-pyrroline-2-carboxylate + NH3 + reduced coenzyme Q1 | - |
? | |
D-Ser + oxidized coenzyme Q1 + H2O | specific activity is 7% of that with D-proline | Helicobacter pylori | 3-hydroxy-2-oxopropanoate + NH3 + reduced coenzyme Q1 | - |
? | |
additional information | activity with D-Val, D-Met, D-Glu, D-Asp, L-Pro and L-Ala is below 5% of the activity with D-Pro | Helicobacter pylori | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, SDS-PAGE | Helicobacter pylori |
? | x * 46040, calculated from sequence | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
DadA | - |
Helicobacter pylori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Helicobacter pylori |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | 25°C: about 50% of maximal activity, 55°C: about 65% of maximal activity | Helicobacter pylori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Helicobacter pylori |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | pH 7.5: about 50% of maximal activity, pH 8.5: about 45% of maximal activity | Helicobacter pylori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoenzyme | Helicobacter pylori |