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Literature summary for 1.4.4.2 extracted from

  • Hasse, D.; Mikkat, S.; Thrun, H.A.; Hagemann, M.; Bauwe, H.
    Properties of recombinant glycine decarboxylase P- and H-protein subunits from the cyanobacterium Synechocystis sp. strain PCC 6803 (2007), FEBS Lett., 581, 1297-1301.
    View publication on PubMed
Search for more literature for 1.4.4.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strains BL21 Gold and LMG194 Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
 lipoylated H-apoprotein P-subunit, at pH 6 Synechocystis sp.
1
-
 lipoylated His-tagged H-apoprotein P-subunit, at pH 6 Synechocystis sp.
2.84
-
 glycine P-subunit in the absesence of H-apoprotein, at pH 6 Synechocystis sp.
2.92
-
 glycine P-subunit in the presence of non-tagged lipoylated H-apoprotein, at pH 8 Synechocystis sp.
2.93
-
 glycine P-subunit in the presence of His-tagged non-lipoylated H-apoprotein, at pH 6 Synechocystis sp.
3.05
-
 glycine P-subunit in the presence of His-tagged lipoylated H-apoprotein, at pH 6 Synechocystis sp.
3.14
-
 glycine P-subunit in the presence of His-tagged lipoylated H-apoprotein, at pH 8 Synechocystis sp.
3.21
-
 glycine P-subunit in the presence of non-tagged lipoylated H-apoprotein, at pH 6 Synechocystis sp.
12.3
-
 His-tagged H-apoprotein P-subunit, at pH 6 Synechocystis sp.
12.3
-
 lipoylated H-apoprotein P-subunit, at pH 8 Synechocystis sp.
12.6
-
 lipoylated His-tagged H-apoprotein P-subunit, at pH 8 Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
 strain PCC 6803
-

Purification (Commentary)

Purification (Comment) Organism
-
 Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycine + His-tagged H-apoprotein very low activity Synechocystis sp. ?
-
 ?
glycine + lipoate very low activity Synechocystis sp. ?
-
 ?
glycine + lipoylated H-apoprotein very low activity Synechocystis sp. ?
-
 ?
glycine + lipoylated His-tagged H-apoprotein very low activity Synechocystis sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
GDC
-
 Synechocystis sp.
glycine decarboxylase
-
 Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 P-subunit Synechocystis sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 P-subunit shows 50% lower activity at pH 8 than at pH 6 Synechocystis sp.