Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
K159Q | the mutation has no effect on the catalytic activity | Escherichia coli |
K159R | the mutation has no effect on the catalytic activity | Escherichia coli |
K72A | the mutant shows a sharp decrease of the catalytic activity compared to the wild type enzyme | Escherichia coli |
K72P | the mutant shows a sharp decrease of the catalytic activity compared to the wild type enzyme | Escherichia coli |
K72Q | the mutant shows a sharp decrease of the catalytic activity compared to the wild type enzyme | Escherichia coli |
K72R | the mutant shows a slight decrease of the catalytic activity compared to the wild type enzyme | Escherichia coli |
General Stability | Organism |
---|---|
acetylation leads to a decrease of the enzymatic activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
pyridoxine 5'-phosphate | wild type enzyme, at pH 8.0 and 37°C | Escherichia coli | |
0.113 | - |
pyridoxine 5'-phosphate | mutant enzyme K72P, at pH 8.0 and 37°C | Escherichia coli | |
0.219 | - |
pyridoxine 5'-phosphate | mutant enzyme K72R, at pH 8.0 and 37°C | Escherichia coli | |
0.625 | - |
pyridoxine 5'-phosphate | mutant enzyme K72A, at pH 8.0 and 37°C | Escherichia coli | |
0.946 | - |
pyridoxine 5'-phosphate | mutant enzyme K72Q, at pH 8.0 and 37°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxine 5'-phosphate + O2 | Escherichia coli | - |
pyridoxal 5'-phosphate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxine 5'-phosphate + O2 | - |
Escherichia coli | pyridoxal 5'-phosphate + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
PNPOx | - |
Escherichia coli |
pyridoxine 5'-phosphate oxidase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
pyridoxine 5'-phosphate | mutant enzyme K72P, at pH 8.0 and 37°C | Escherichia coli | |
0.0004 | - |
pyridoxine 5'-phosphate | mutant enzyme K72A, at pH 8.0 and 37°C | Escherichia coli | |
0.0015 | - |
pyridoxine 5'-phosphate | mutant enzyme K72Q, at pH 8.0 and 37°C | Escherichia coli | |
0.022 | - |
pyridoxine 5'-phosphate | wild type enzyme, at pH 8.0 and 37°C | Escherichia coli | |
0.024 | - |
pyridoxine 5'-phosphate | mutant enzyme K72R, at pH 8.0 and 37°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Escherichia coli |