Any feedback?
Literature summary for 1.4.3.5 extracted from
- Purification and characterization of the pyridoxol-5'-phosphate:oxygen oxidoreductase (deaminating) from Escherichia coli (1995), Biochim. Biophys. Acta, 1247, 265-271.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli as maltose binding protein fusion | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.368 | - |
pyridoxal 5'-phosphate | recombinant enzyme after cleavage from fusion protein | Escherichia coli | |
| 0.4 | - |
pyridoxamine 5'-phosphate | recombinant enzyme after cleavage from fusion protein | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25500 | - |
recombinant enzyme after cleavage from fusion protein | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyridoxamine 5'-phosphate + H2O + O2 | - |
Escherichia coli | pyridoxal 5'-phosphate + NH3 + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 25500, recombinant enzyme after cleavage from fusion protein, gel filtration | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 28 | - |
pyridoxamine 5'-phosphate | recombinant enzyme after cleavage from fusion protein | Escherichia coli | |
| 33 | - |
pyridoxal 5'-phosphate | recombinant enzyme after cleavage from fusion protein | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
recombinant enzyme after cleavage from fusion protein | Escherichia coli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
pyridoxal 5'-phosphate | - |
Escherichia coli | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
