Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
using 12% (w/v) polyethylene glycol 4000, 100 mM N-(2-acetamido)-2-iminodiacetic acid buffer pH 6.5, and 70 mM lithium sulfate | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
I199A | the mutant exhibits catalytic properties with 4fold increased amine Km but unaltered kcat values | Homo sapiens |
I199A/Y326A | the mutant exhibits catalytic properties with 75fold increased amine Km but unaltered kcat values. The mutant shows inhibitor binding properties more similar to those of isoform MAO A than to isoform MAO B. Benzylamine is a poor substrate for the double mutant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,4-diphenyl-1,3-butadiene | - |
Homo sapiens | |
1,4-diphenyl-2-butene | - |
Homo sapiens | |
8-(3-chloro)styrylcaffeine | - |
Homo sapiens | |
farnesol | - |
Homo sapiens | |
Harmane | - |
Homo sapiens | |
isatin | non-specific reversible inhibitor | Homo sapiens | |
methylene blue | - |
Homo sapiens | |
additional information | isoform MAO B is not inhibited by pirlindole | Homo sapiens | |
safinamide | - |
Homo sapiens | |
tranylcypromine | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0089 | - |
phenylbutylamine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.0094 | - |
Phenylethylamine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.019 | - |
phenylbutylamine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.027 | - |
kynuramine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.04 | - |
Phenylethylamine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.055 | - |
phenylbutylamine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.094 | - |
kynuramine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.15 | - |
benzylamine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.289 | - |
kynuramine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.703 | - |
Phenylethylamine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
2.133 | - |
benzylamine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-[4-(trifluoromethyl)phenyl]methanamine + H2O + O2 | - |
Homo sapiens | 4-(trifluoromethyl)benzaldehyde + NH3 + H2O2 | - |
? | |
benzylamine + H2O + O2 | - |
Homo sapiens | benzaldehyde + NH3 + H2O2 | - |
? | |
kynuramine + H2O + O2 | - |
Homo sapiens | 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2 | - |
? | |
phenylbutylamine + H2O + O2 | - |
Homo sapiens | 2-phenylbutanal + NH3 + H2O2 + NH3 + H2O2 | - |
? | |
phenylethylamine + H2O + O2 | - |
Homo sapiens | 2-phenylethanal + NH3 + H2O2 + NH3 + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MAO B | isoform | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.07 | - |
kynuramine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.47 | - |
kynuramine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.6 | - |
kynuramine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.83 | - |
Phenylethylamine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.83 | - |
phenylbutylamine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
2.05 | - |
Phenylethylamine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
2.25 | - |
phenylbutylamine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
2.34 | - |
phenylbutylamine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
2.87 | - |
Phenylethylamine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
3.8 | - |
benzylamine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
5 | - |
benzylamine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000021 | - |
safinamide | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.000143 | - |
methylene blue | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.000214 | - |
methylene blue | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.00027 | - |
8-(3-chloro)styrylcaffeine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.0005 | - |
safinamide | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.001 | - |
1,4-diphenyl-1,3-butadiene | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.00101 | - |
methylene blue | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.0017 | - |
8-(3-chloro)styrylcaffeine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.0023 | - |
farnesol | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.0027 | - |
farnesol | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.003 | - |
isatin | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.004 | - |
safinamide | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.007 | - |
1,4-diphenyl-1,3-butadiene | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.0081 | - |
1,4-diphenyl-2-butene | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.011 | - |
tranylcypromine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.012 | - |
isatin | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.016 | - |
tranylcypromine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.017 | - |
tranylcypromine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.018 | - |
farnesol | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.0345 | - |
1,4-diphenyl-2-butene | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.14 | - |
Harmane | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.165 | - |
Harmane | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.36 | - |
isatin | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
0.55 | - |
Harmane | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.78 | - |
benzylamine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
2.7 | - |
Phenylethylamine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
3.7 | - |
kynuramine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
15.6 | - |
kynuramine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
33.3 | - |
benzylamine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
43.3 | - |
phenylbutylamine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
51.7 | - |
Phenylethylamine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
59.2 | - |
kynuramine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
96.7 | - |
phenylbutylamine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
253.3 | - |
phenylbutylamine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
300 | - |
Phenylethylamine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens |