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Literature summary for 1.4.3.22 extracted from

  • Vianello, F.; Di Paolo, M.L.; Stevanato, R.; Gasparini, R.; Rigo, A.
    Purification and characterization of amine oxidase from soybean seedlings (1993), Arch. Biochem. Biophys., 307, 35-39.
    View publication on PubMed

General Stability

General Stability Organism
unstable at low ionic strenght, half-life of 8 d in 5 mM potassium phosphate, pH 7.0, at 4°C, 33 d if 100 mM NaCl is added, unstable if 0.01% n-octyl-beta-D-glucopyranoside or 0.1% Triton X-100 are added, loss of 25% activity after 5 d Glycine max

Inhibitors

Inhibitors Comment Organism Structure
1,4-diaminocyclohexane 5 mM, 69% inhibition Glycine max
aminoguanidine
-
Glycine max
aniline 10 mM, 43% inhibition Glycine max
cyanide
-
Glycine max
Cyclohexylamine 10 mM, 93% inhibition Glycine max
Dicyclohexylamine 10 mM, 79% inhibition Glycine max
diethyldithiocarbamate
-
Glycine max
piperidine 10 mM, 55% inhibition Glycine max
Semicarbazide
-
Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.065
-
cadaverine
-
Glycine max
0.19
-
putrescine
-
Glycine max
5.9
-
spermidine
-
Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77000
-
x * 77000, SDS-PAGE Glycine max
113000
-
gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein heterogenity may be due to carbohydrate moiety Glycine max

Purification (Commentary)

Purification (Comment) Organism
-
Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
263
-
-
Glycine max

Storage Stability

Storage Stability Organism
-80°C, 5 mM potassium phosphate, pH 7.0, 100 mM NaCl, 2 months, no loss of activity Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-diaminobutane + H2O + O2
-
Glycine max 4-aminobutanal + NH3 + H2O2
-
?
cadaverine + H2O + O2
-
Glycine max 5-aminopentanal + NH3 + H2O2
-
r
RCH2NH2 + H2O + O2
-
Glycine max RCHO + NH3 + H2O2
-
?
spermidine + H2O + O2
-
Glycine max ?
-
?

Subunits

Subunits Comment Organism
? x * 77000, SDS-PAGE Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
163
-
putrescine
-
Glycine max
193
-
spermidine
-
Glycine max
498
-
cadaverine
-
Glycine max

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7 slight decrease in activity, complete loss at pH 4 and pH 8 Glycine max

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.045
-
Cyclohexylamine
-
Glycine max
0.78
-
Dicyclohexylamine
-
Glycine max
1.2
-
piperidine
-
Glycine max
5
-
aniline
-
Glycine max