Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Paenarthrobacter aurescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Phenylethylamine | substrate inhibition | Paenarthrobacter aurescens | |
histamine | substrate inhibition; substrate inhibition | Paenarthrobacter aurescens | |
additional information | the enzyme activity is not significantly affected in the presence of 5 mM EDTA; the enzyme activity is not significantly affected in the presence of 5 mM EDTA | Paenarthrobacter aurescens | |
tyramine | substrate inhibition; substrate inhibition | Paenarthrobacter aurescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0055 | - |
2-Phenylethylamine | isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
0.012 | - |
2-Phenylethylamine | isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
0.02 | - |
tyramine | isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
0.03 | - |
tyramine | isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
0.41 | - |
histamine | isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
0.88 | - |
histamine | isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
7.04 | - |
spermidine | isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
12.7 | - |
cadaverine | isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | required for activity, after overnight incubation with 0.1 mM CuSO4, isoform AMAO3 shows slightly enhanced activity (114% of untreated enzyme activity) | Paenarthrobacter aurescens | |
Cu2+ | required for activity, after overnight incubation with 0.1 mM CuSO4, there is no change in the activity of isoform AMAO2 | Paenarthrobacter aurescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenarthrobacter aurescens | A1R2C3 | isoform AMAO2 | - |
Paenarthrobacter aurescens | A1RDD3 | isoform AMAO3 | - |
Paenarthrobacter aurescens TC1 | A1R2C3 | isoform AMAO2 | - |
Paenarthrobacter aurescens TC1 | A1RDD3 | isoform AMAO3 | - |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Paenarthrobacter aurescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenylethylamine + H2O + O2 | - |
Paenarthrobacter aurescens | ? | - |
? | |
2-phenylethylamine + H2O + O2 | best substrate | Paenarthrobacter aurescens | phenylacetaldehyde + NH3 + H2O2 | - |
? | |
cadaverine + H2O + O2 | - |
Paenarthrobacter aurescens | ? | - |
? | |
cadaverine + H2O + O2 | - |
Paenarthrobacter aurescens TC1 | ? | - |
? | |
histamine + H2O + O2 | - |
Paenarthrobacter aurescens | ? | - |
? | |
histamine + H2O + O2 | - |
Paenarthrobacter aurescens TC1 | ? | - |
? | |
putrescine + H2O + O2 | - |
Paenarthrobacter aurescens | ? | - |
? | |
putrescine + H2O + O2 | - |
Paenarthrobacter aurescens TC1 | ? | - |
? | |
spermidine + H2O + O2 | - |
Paenarthrobacter aurescens | ? | - |
? | |
spermidine + H2O + O2 | - |
Paenarthrobacter aurescens TC1 | ? | - |
? | |
tyramine + H2O + O2 | - |
Paenarthrobacter aurescens | (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2 | - |
? | |
tyramine + H2O + O2 | - |
Paenarthrobacter aurescens TC1 | (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AMAO2 | isoform | Paenarthrobacter aurescens |
AMAO3 | isoform | Paenarthrobacter aurescens |
copper-containing monoamine oxidase | - |
Paenarthrobacter aurescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
isoform AMAO3 | Paenarthrobacter aurescens |
55 | - |
isoform AMAO2 | Paenarthrobacter aurescens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0429 | - |
spermidine | isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
0.0619 | - |
cadaverine | isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
0.46 | - |
histamine | isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
1.6 | - |
tyramine | isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
1.72 | - |
2-Phenylethylamine | isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
3.95 | - |
tyramine | isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
5.12 | - |
2-Phenylethylamine | isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
5.91 | - |
histamine | isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
isoform AMAO2 | Paenarthrobacter aurescens |
7 | - |
isoform AMAO3 | Paenarthrobacter aurescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2,4,5-trihydroxyphenylalanine quinone | - |
Paenarthrobacter aurescens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.84 | - |
2-Phenylethylamine | isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
1.08 | - |
tyramine | isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
1.41 | - |
2-Phenylethylamine | isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
2.93 | - |
tyramine | isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
3.51 | - |
histamine | isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
putrescine | isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
0.52 | - |
histamine | isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
14.6 | - |
histamine | isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
82.3 | - |
tyramine | isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
133 | - |
tyramine | isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
314 | - |
2-Phenylethylamine | isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens | |
429 | - |
2-Phenylethylamine | isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C | Paenarthrobacter aurescens |