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Literature summary for 1.4.3.21 extracted from

  • Lee, J.I.; Kim, Y.W.
    Characterization of amine oxidases from Arthrobacter aurescens and application for determination of biogenic amines (2013), World J. Microbiol. Biotechnol., 29, 673-682.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Paenarthrobacter aurescens

Inhibitors

Inhibitors Comment Organism Structure
2-Phenylethylamine substrate inhibition Paenarthrobacter aurescens
histamine substrate inhibition; substrate inhibition Paenarthrobacter aurescens
additional information the enzyme activity is not significantly affected in the presence of 5 mM EDTA; the enzyme activity is not significantly affected in the presence of 5 mM EDTA Paenarthrobacter aurescens
tyramine substrate inhibition; substrate inhibition Paenarthrobacter aurescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0055
-
2-Phenylethylamine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
0.012
-
2-Phenylethylamine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
0.02
-
tyramine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
0.03
-
tyramine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
0.41
-
histamine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
0.88
-
histamine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
7.04
-
spermidine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
12.7
-
cadaverine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ required for activity, after overnight incubation with 0.1 mM CuSO4, isoform AMAO3 shows slightly enhanced activity (114% of untreated enzyme activity) Paenarthrobacter aurescens
Cu2+ required for activity, after overnight incubation with 0.1 mM CuSO4, there is no change in the activity of isoform AMAO2 Paenarthrobacter aurescens

Organism

Organism UniProt Comment Textmining
Paenarthrobacter aurescens A1R2C3 isoform AMAO2
-
Paenarthrobacter aurescens A1RDD3 isoform AMAO3
-
Paenarthrobacter aurescens TC1 A1R2C3 isoform AMAO2
-
Paenarthrobacter aurescens TC1 A1RDD3 isoform AMAO3
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Paenarthrobacter aurescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phenylethylamine + H2O + O2
-
Paenarthrobacter aurescens ?
-
?
2-phenylethylamine + H2O + O2 best substrate Paenarthrobacter aurescens phenylacetaldehyde + NH3 + H2O2
-
?
cadaverine + H2O + O2
-
Paenarthrobacter aurescens ?
-
?
cadaverine + H2O + O2
-
Paenarthrobacter aurescens TC1 ?
-
?
histamine + H2O + O2
-
Paenarthrobacter aurescens ?
-
?
histamine + H2O + O2
-
Paenarthrobacter aurescens TC1 ?
-
?
putrescine + H2O + O2
-
Paenarthrobacter aurescens ?
-
?
putrescine + H2O + O2
-
Paenarthrobacter aurescens TC1 ?
-
?
spermidine + H2O + O2
-
Paenarthrobacter aurescens ?
-
?
spermidine + H2O + O2
-
Paenarthrobacter aurescens TC1 ?
-
?
tyramine + H2O + O2
-
Paenarthrobacter aurescens (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2
-
?
tyramine + H2O + O2
-
Paenarthrobacter aurescens TC1 (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2
-
?

Synonyms

Synonyms Comment Organism
AMAO2 isoform Paenarthrobacter aurescens
AMAO3 isoform Paenarthrobacter aurescens
copper-containing monoamine oxidase
-
Paenarthrobacter aurescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
isoform AMAO3 Paenarthrobacter aurescens
55
-
isoform AMAO2 Paenarthrobacter aurescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0429
-
spermidine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
0.0619
-
cadaverine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
0.46
-
histamine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.6
-
tyramine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.72
-
2-Phenylethylamine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
3.95
-
tyramine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
5.12
-
2-Phenylethylamine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
5.91
-
histamine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
isoform AMAO2 Paenarthrobacter aurescens
7
-
isoform AMAO3 Paenarthrobacter aurescens

Cofactor

Cofactor Comment Organism Structure
2,4,5-trihydroxyphenylalanine quinone
-
Paenarthrobacter aurescens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.84
-
2-Phenylethylamine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.08
-
tyramine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
1.41
-
2-Phenylethylamine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
2.93
-
tyramine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
3.51
-
histamine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.15
-
putrescine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
0.52
-
histamine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
14.6
-
histamine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
82.3
-
tyramine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
133
-
tyramine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
314
-
2-Phenylethylamine isoform AMAO3, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens
429
-
2-Phenylethylamine isoform AMAO2, in 100 mM potassium phosphate buffer (pH 7.0), at 37°C Paenarthrobacter aurescens