Protein Variants | Comment | Organism |
---|---|---|
D383E | turnover-number of mutant enzyme is reduced up to 2000fold, depending on pH-value | Escherichia coli |
D383N | catalytically inactive mutant enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tranylcypromine | fully reversible competitive onhibitor | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00088 | - |
beta-phenylethylamine | pH 8.0, mutant enzyme D383E | Escherichia coli | |
0.0012 | - |
beta-phenylethylamine | pH 7.0, wild-type enzyme | Escherichia coli | |
0.0017 | - |
beta-phenylethylamine | pH 7.5, wild-type enzyme | Escherichia coli | |
0.0017 | - |
beta-phenylethylamine | pH 6.5, wild-type enzyme | Escherichia coli | |
0.0018 | - |
beta-phenylethylamine | pH 6.0, wild-type enzyme | Escherichia coli | |
0.0023 | - |
beta-phenylethylamine | pH 8.0, wild-type enzyme | Escherichia coli | |
0.0023 | - |
beta-phenylethylamine | pH 5.75, wild-type enzyme | Escherichia coli | |
0.00247 | - |
beta-phenylethylamine | pH 7.0, mutant enzyme D383E | Escherichia coli | |
0.0078 | - |
beta-phenylethylamine | pH 5.5, wild-type enzyme | Escherichia coli | |
0.00962 | - |
beta-phenylethylamine | pH 6.0, mutant enzyme D383E | Escherichia coli | |
0.028 | - |
beta-phenylethylamine | pH 5.5, mutant enzyme D383E | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | contains one Cu2+ per monomer | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0038 | - |
mutant enzyme D383E | Escherichia coli |
11 | - |
wild-type enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenylethylamine + H2O + O2 | - |
Escherichia coli | 2-phenylethanal + NH3 + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ECAO | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00612 | - |
beta-phenylethylamine | pH 8.0, mutant enzyme D383E | Escherichia coli | |
0.00937 | - |
beta-phenylethylamine | pH 7.0, mutant enzyme D383E | Escherichia coli | |
0.01163 | - |
beta-phenylethylamine | pH 5.5, mutant enzyme D383E | Escherichia coli | |
0.012 | - |
beta-phenylethylamine | pH 6.0, mutant enzyme D383E | Escherichia coli | |
9.6 | - |
beta-phenylethylamine | pH 5.5, wild-type enzyme | Escherichia coli | |
11.45 | - |
beta-phenylethylamine | pH 5.75, wild-type enzyme | Escherichia coli | |
13.32 | - |
beta-phenylethylamine | pH 7.5, wild-type enzyme | Escherichia coli | |
13.68 | - |
beta-phenylethylamine | pH 8.0, wild-type enzyme | Escherichia coli | |
14.98 | - |
beta-phenylethylamine | pH 7.0, wild-type enzyme | Escherichia coli | |
20.7 | - |
beta-phenylethylamine | pH 6.0, wild-type enzyme | Escherichia coli | |
20.77 | - |
beta-phenylethylamine | pH 6.5, wild-type enzyme | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2,4,5-trihydroxyphenylalanine quinone | enzyme contains one per monomer | Escherichia coli |