Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)CodonPlus-RIL cells | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | 22% residual activity after 30 min incubation at 30% (v/v) | Bacillus subtilis | |
acetonitrile | complete inhibition at 30% (v/v) | Bacillus subtilis | |
Co2+ | there is a sharp decrease in activity when 1 mM Co2+ is added to the reaction assay | Bacillus subtilis | |
diethylether | 73% residual activity after 30 min incubation at 30% (v/v) | Bacillus subtilis | |
ethyl acetate | 19% residual activity after 30 min incubation at 30% (v/v) | Bacillus subtilis | |
methanol | 54% residual activity after 30 min incubation at 30% (v/v) | Bacillus subtilis | |
N,N-Dimethyl formamide | 17% residual activity after 30 min incubation at 30% (v/v) | Bacillus subtilis | |
Zn2+ | there is a sharp decrease in activity when 1 mM Zn2+ is added to the reaction assay | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
glycine | at pH 8.0 and 50°C | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no enhancement of enzyme activity in the presence of metal cations | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40000, SDS-PAGE | Bacillus subtilis |
40760 | - |
4 * 40760, MALDI-TOF mass spectrometry | Bacillus subtilis |
40761 | - |
4 * 40761, calculated from amino acid sequence | Bacillus subtilis |
160000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine + H2O + O2 | Bacillus subtilis | - |
pyruvate + NH3 + H2O2 | - |
? | |
D-alanine + H2O + O2 | Bacillus subtilis R5 | - |
pyruvate + NH3 + H2O2 | - |
? | |
glycine + H2O + O2 | Bacillus subtilis | - |
glyoxylate + NH3 + H2O2 | - |
r | |
glycine + H2O + O2 | Bacillus subtilis R5 | - |
glyoxylate + NH3 + H2O2 | - |
r | |
additional information | Bacillus subtilis | no activity with L-alanine | ? | - |
? | |
additional information | Bacillus subtilis R5 | no activity with L-alanine | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | D7UTZ8 | - |
- |
Bacillus subtilis R5 | D7UTZ8 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Resource Q column chromatography and Resource ISO column chromatography | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14 | - |
cell free extract, when glycine is used as a substrate, at pH 8.0 and 37°C | Bacillus subtilis |
960 | - |
after 68.6fold purification, when glycine is used as a substrate, at pH 8.0 and 37°C | Bacillus subtilis |
1000 | - |
after 68.6fold purification, when D-alanine is used as a substrate, at pH 8.0 and 37°C | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine + H2O + O2 | - |
Bacillus subtilis | pyruvate + NH3 + H2O2 | - |
? | |
D-alanine + H2O + O2 | - |
Bacillus subtilis R5 | pyruvate + NH3 + H2O2 | - |
? | |
glycine + H2O + O2 | - |
Bacillus subtilis | glyoxylate + NH3 + H2O2 | - |
r | |
glycine + H2O + O2 | - |
Bacillus subtilis R5 | glyoxylate + NH3 + H2O2 | - |
r | |
additional information | no activity with L-alanine | Bacillus subtilis | ? | - |
? | |
additional information | no activity with L-alanine | Bacillus subtilis R5 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 40000, SDS-PAGE | Bacillus subtilis |
homotetramer | 4 * 40760, MALDI-TOF mass spectrometry | Bacillus subtilis |
homotetramer | 4 * 40761, calculated from amino acid sequence | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
glycine:oxygen oxidoreductase (deaminating) | - |
Bacillus subtilis |
GoxR | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 55 | the enzyme exhibits almost 80% of its activity at temperatures 40 and 55°C. The activity sharply decreases above 55°C | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | about 75% activity at pH 7.0, more than 50% activity is retained between pH 7.5 and 8.5, about 60% activity at pH 9.0. The enzyme activity decreases sharply below pH 7.0 and above pH 9.0. There is a negligible amount of activity at pH below 5.0 and above 10.0 | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Bacillus subtilis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus subtilis | calculated from amino acid sequence | - |
5.8 |