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Literature summary for 1.4.3.19 extracted from

  • Jamil, F.; Rashid, N.; Gardner, Q.; Akhtar, M.
    Gene cloning and characterization of glycine oxidase from newly isolated Bacillus subtilis strain R5 (2011), Biologia, 66, 1-7.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)CodonPlus-RIL cells Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
acetone 22% residual activity after 30 min incubation at 30% (v/v) Bacillus subtilis
acetonitrile complete inhibition at 30% (v/v) Bacillus subtilis
Co2+ there is a sharp decrease in activity when 1 mM Co2+ is added to the reaction assay Bacillus subtilis
diethylether 73% residual activity after 30 min incubation at 30% (v/v) Bacillus subtilis
ethyl acetate 19% residual activity after 30 min incubation at 30% (v/v) Bacillus subtilis
methanol 54% residual activity after 30 min incubation at 30% (v/v) Bacillus subtilis
N,N-Dimethyl formamide 17% residual activity after 30 min incubation at 30% (v/v) Bacillus subtilis
Zn2+ there is a sharp decrease in activity when 1 mM Zn2+ is added to the reaction assay Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
glycine at pH 8.0 and 50°C Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no enhancement of enzyme activity in the presence of metal cations Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
4 * 40000, SDS-PAGE Bacillus subtilis
40760
-
4 * 40760, MALDI-TOF mass spectrometry Bacillus subtilis
40761
-
4 * 40761, calculated from amino acid sequence Bacillus subtilis
160000
-
gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-alanine + H2O + O2 Bacillus subtilis
-
pyruvate + NH3 + H2O2
-
?
D-alanine + H2O + O2 Bacillus subtilis R5
-
pyruvate + NH3 + H2O2
-
?
glycine + H2O + O2 Bacillus subtilis
-
glyoxylate + NH3 + H2O2
-
r
glycine + H2O + O2 Bacillus subtilis R5
-
glyoxylate + NH3 + H2O2
-
r
additional information Bacillus subtilis no activity with L-alanine ?
-
?
additional information Bacillus subtilis R5 no activity with L-alanine ?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis D7UTZ8
-
-
Bacillus subtilis R5 D7UTZ8
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Resource Q column chromatography and Resource ISO column chromatography Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14
-
cell free extract, when glycine is used as a substrate, at pH 8.0 and 37°C Bacillus subtilis
960
-
after 68.6fold purification, when glycine is used as a substrate, at pH 8.0 and 37°C Bacillus subtilis
1000
-
after 68.6fold purification, when D-alanine is used as a substrate, at pH 8.0 and 37°C Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-alanine + H2O + O2
-
Bacillus subtilis pyruvate + NH3 + H2O2
-
?
D-alanine + H2O + O2
-
Bacillus subtilis R5 pyruvate + NH3 + H2O2
-
?
glycine + H2O + O2
-
Bacillus subtilis glyoxylate + NH3 + H2O2
-
r
glycine + H2O + O2
-
Bacillus subtilis R5 glyoxylate + NH3 + H2O2
-
r
additional information no activity with L-alanine Bacillus subtilis ?
-
?
additional information no activity with L-alanine Bacillus subtilis R5 ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 40000, SDS-PAGE Bacillus subtilis
homotetramer 4 * 40760, MALDI-TOF mass spectrometry Bacillus subtilis
homotetramer 4 * 40761, calculated from amino acid sequence Bacillus subtilis

Synonyms

Synonyms Comment Organism
glycine:oxygen oxidoreductase (deaminating)
-
Bacillus subtilis
GoxR
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 55 the enzyme exhibits almost 80% of its activity at temperatures 40 and 55°C. The activity sharply decreases above 55°C Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
7 9 about 75% activity at pH 7.0, more than 50% activity is retained between pH 7.5 and 8.5, about 60% activity at pH 9.0. The enzyme activity decreases sharply below pH 7.0 and above pH 9.0. There is a negligible amount of activity at pH below 5.0 and above 10.0 Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
FAD
-
Bacillus subtilis

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus subtilis calculated from amino acid sequence
-
5.8