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Literature summary for 1.4.3.14 extracted from

  • Endo, H.; Hayashi, Y.; Kitani, Y.; Ren, H.; Hayashi, T.; Nagashima, Y.
    Optical enzyme sensor for determining L-lysine content using L-lysine oxidase from the rockfish Sebastes schlegeli (2008), Anal. Bioanal. Chem., 391, 1255-1261.
    View publication on PubMed
Search for more literature for 1.4.3.14

Application

Application Comment Organism
nutrition the optical enzyme sensor system with immobilized LyOx membrane can be used for rapid determination of L-Lys in a real food sample Trichoderma viride
nutrition the optical enzyme sensor system with immobilized LyOx membrane can be used for rapid determination of L-Lys in a real food sample Sebastes schlegelii

Organism

Organism UniProt Comment Textmining
Sebastes schlegelii
-
-
-
Trichoderma viride
-
-
-

Purification (Commentary)

Purification (Comment) Organism
lectin affinity column chromatography, anion-exchange HPLC, and hydroxyapatite column chromatography Trichoderma viride
lectin affinity column chromatography, anion-exchange HPLC, and hydroxyapatite column chromatography Sebastes schlegelii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + O2 + H2O
-
 Sebastes schlegelii 6-amino-2-oxohexanoate + NH3 + H2O2
-
 ?
additional information commercially manufactured LyOx is generally not highly selective for L-Lys among amino acids Trichoderma viride ?
-
 ?

Synonyms

Synonyms Comment Organism
L6150 commercial preparation Trichoderma viride
LyOx
-
 Trichoderma viride
LyOx
-
 Sebastes schlegelii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.8 membrane-immobilized enzyme Trichoderma viride
7 7.8 membrane-immobilized enzyme Sebastes schlegelii

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Trichoderma viride
FAD
-
 Sebastes schlegelii