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Literature summary for 1.4.3.13 extracted from
- Purification of high yields of catalytically active lysyl oxidase directly from Escherichia coli cell culture (2010), Protein Expr. Purif., 74, 116-121.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning from cDNA library, DNA and amino acid sequence determination and analysis, expression of C-terminally His6-tagged LOX in Escherichia coli | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-aminopropionitrile | LOX activity is inhibited by 0.5 mM and above concentrations | Homo sapiens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | dependent on, copper concentration is 0.0052 mM corresponding to 19.3% | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
x * 29000, active mature protein | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
isoenzymes LOXL, LOXL2, LOXL3, and LOXL4 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | lysyl oxidase is synthesized and exported to the extracellular space as a 50 kDa inactive preproprotein. In the extracellular space, it is cleaved at the specific Gly168-Asp169 by procollagen C-proteinase to yield the active 29 kDa enzyme | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| lysyl oxidase is a highly insoluble enzyme requiring high concentrations of urea to solubilize. Development and optimization of a method to obtain lysyl oxidase in high yields directly from an Escherichia coli culture without the need for refolding of inclusion bodies using nutrient rich media, overview | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| aorta | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.31 | - |
purified recombinant enzyme, pH not specified in the publication, 37°C | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzymatic activity is assayed using a peroxide-coupled assay and the nonpeptidyl substrate 1,5-diaminopentane producing the corresponding aldehyde | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29000, active mature protein | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LOX | - |
Homo sapiens |
| lysyl oxidase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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