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Literature summary for 1.4.3.10 extracted from

  • Boka, B.; Adanyi, N.; Szamos, J.; Virag, D.; Kiss, A.
    Putrescine biosensor based on putrescine oxidase from Kocuria rosea (2012), Enzyme Microb. Technol., 51, 258-262.
    View publication on PubMed
Search for more literature for 1.4.3.10

Application

Application Comment Organism
food industry the immobilized enzyme may be used as a sensor for analysis of beer samples Kocuria rosea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 2 * 50000, SDS-PAGE Kocuria rosea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
putrescine + O2 + H2O Kocuria rosea 100% activity 4-aminobutanal + NH3 + H2O2
-
 ?
putrescine + O2 + H2O Kocuria rosea K22 100% activity 4-aminobutanal + NH3 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Kocuria rosea
-
 formerly Micrococcus rubens
-
Kocuria rosea K22
-
 formerly Micrococcus rubens
-

Purification (Commentary)

Purification (Comment) Organism
three-phase partitioning and DEAE Sephadex gel filtration Kocuria rosea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cadaverine + O2 + H2O 10.7% activity compared to putrescine Kocuria rosea 5-aminopentanal + NH3 + H2O2
-
 ?
cadaverine + O2 + H2O 10.7% activity compared to putrescine Kocuria rosea K22 5-aminopentanal + NH3 + H2O2
-
 ?
histamine + O2 + H2O 1.7% activity compared to putrescine Kocuria rosea 1H-imidazol-4-ylacetaldehyde + NH3 + H2O2
-
 ?
histamine + O2 + H2O 1.7% activity compared to putrescine Kocuria rosea K22 1H-imidazol-4-ylacetaldehyde + NH3 + H2O2
-
 ?
putrescine + O2 + H2O 100% activity Kocuria rosea 4-aminobutanal + NH3 + H2O2
-
 ?
putrescine + O2 + H2O 100% activity Kocuria rosea K22 4-aminobutanal + NH3 + H2O2
-
 ?
spermidine + O2 + H2O 5.0% activity compared to putrescine Kocuria rosea 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
-
 ?
spermidine + O2 + H2O 5.0% activity compared to putrescine Kocuria rosea K22 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
-
 ?
tryptamine + O2 + H2O 5.2% activity compared to putrescine Kocuria rosea 1H-indol-3-yl-acetaldehyde + NH3 + H2O2
-
 ?
tyramine + O2 + H2O 2.0% activity compared to putrescine Kocuria rosea (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2
-
 ?
tyramine + O2 + H2O 2.0% activity compared to putrescine Kocuria rosea K22 (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 50000, SDS-PAGE Kocuria rosea

Synonyms

Synonyms Comment Organism
PUOX
-
 Kocuria rosea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.6
-
 Kocuria rosea

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Kocuria rosea