Application | Comment | Organism |
---|---|---|
analysis | specific measurement of putrescine with putrescine oxidase and aminobutyraldehyde dehydrogenase | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
putrescine | substrate inhibition at 10 mM or above | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
putrescine | - |
Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
-20°C, in presence of 25% glycerol, 1 year, stable | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cadaverine + O2 + H2O | at 11% of the activity with putrescine | Pseudomonas putida | 5-aminopentanal + NH3 + H2O2 | - |
? | |
putrescine + O2 + H2O | - |
Pseudomonas putida | 4-aminobutanal + NH3 + H2O2 | - |
? | |
spermidine + O2 + H2O | at 9% of the activity with putrescine | Pseudomonas putida | 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
pH 7.5, 20 min, 17% loss of activity | Pseudomonas putida |
50 | - |
pH 7.5, 20 min, 62% loss of activity | Pseudomonas putida |
60 | - |
pH 7.5, 20 min, 93% loss of activity | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 11.7 | - |
Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10.5 | stable | Pseudomonas putida |