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Literature summary for 1.4.3.1 extracted from
- Cloning and expression in Escherichia coli of the D-aspartate oxidase gene from the yeast Cryptococcus humicola and characterization of the recombinant enzyme (2004), J. Biochem., 135, 533-540.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Vanrija humicola |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-malate | 33% inhibition | Vanrija humicola |  |
| malonate | 60% inhibition | Vanrija humicola | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.9 | - |
D-Aspartate | 37°C, pH 7.5 | Vanrija humicola | |
| 62.6 | - |
D-glutamate | 37°C, pH 7.5 | Vanrija humicola | |
| 85.3 | - |
N-Methyl-D-aspartate | 37°C, pH 7.5 | Vanrija humicola | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39459 | - |
4 * 39459, deduced from nucleotide sequence | Vanrija humicola |
| 40000 | - |
4 * 40000, SDS-PAGE | Vanrija humicola |
| 164000 | - |
gel filtration | Vanrija humicola |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vanrija humicola | Q75WF1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant chDDO, ammonium sulfate, Butyl-Sepharose, SP-Sepharose | Vanrija humicola |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 123 | - |
- |
Vanrija humicola |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-aspartate + H2O + O2 | - |
Vanrija humicola | oxaloacetate + NH3 + H2O2 | - |
? | |
| D-glutamate + H2O + O2 | - |
Vanrija humicola | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
| N-methyl-D-aspartate + H2O + O2 | - |
Vanrija humicola | oxaloacetate + methylamine + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 40000, SDS-PAGE | Vanrija humicola |
| tetramer | 4 * 39459, deduced from nucleotide sequence | Vanrija humicola |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ChDDO | - |
Vanrija humicola |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Vanrija humicola |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
little loss of activity | Vanrija humicola |
| 60 | - |
complete loss of activity | Vanrija humicola |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
D-glutamate | 37°C, pH 7.5 | Vanrija humicola | |
| 38.1 | - |
N-Methyl-D-aspartate | 37°C, pH 7.5 | Vanrija humicola | |
| 82.6 | - |
D-Aspartate | 37°C, pH 7.5 | Vanrija humicola | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Vanrija humicola |
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