Literature summary for 1.4.1.9 extracted from

Oikawa, T.; Kataoka, K.; Jin, Y.; Suzuki, S.; Soda, K.
Fragmentary form of thermostable leucine dehydrogenase of Bacillus stearothermophilus: its construction and reconstitution of active fragmentary enzyme (2001), Biochem. Biophys. Res. Commun., 280, 1177-1182.

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Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a fragmentary enzyme form consisting of an N-terminal polypeptide fragment corresponding to the substrate-binding domain including an N-terminus, and a C-terminal fragment corresponding to the NAD+-binding domain	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Leu + H2O + NAD+	-	Geobacillus stearothermophilus	4-methyl-2-oxopentanoate + NH3 + NADH	-	r

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	60 min, enzyme retains more than 75% of its activity	Geobacillus stearothermophilus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Geobacillus stearothermophilus
NADH	-	Geobacillus stearothermophilus

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Release 2023.1 (January 2023)