KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
L-glutamine | in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Proteus sp. | |
0.025 | - |
L-isoleucine | in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Citrobacter sp. | |
0.034 | - |
L-methionine | in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Proteus sp. | |
0.044 | - |
L-valine | in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter sp. | - |
- |
- |
Proteus sp. | - |
- |
- |
Pseudomonas sp. | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.47 | - |
using L-valine, L-cysteine, L-serine, or L-glutamine as substrate, in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Pseudomonas sp. |
0.7 | - |
using L-glutamine as substrate, in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Citrobacter sp. |
0.73 | - |
using L-cysteine, L-serine, or L-glutamine as substrate, in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Proteus sp. |
1.2 | - |
using L-methionine as substrate, in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Proteus sp. |
1.59 | - |
using L-isoleucine as substrate, in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Citrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + H2O + NAD+ | - |
Pseudomonas sp. | 3-mercapto-2-oxopropanoate + NH3 + NADH | - |
? | |
L-cysteine + H2O + NAD+ | - |
Citrobacter sp. | 3-mercapto-2-oxopropanoate + NH3 + NADH | - |
? | |
L-cysteine + H2O + NAD+ | - |
Proteus sp. | 3-mercapto-2-oxopropanoate + NH3 + NADH | - |
? | |
L-glutamine + H2O + NAD+ | - |
Pseudomonas sp. | 2-oxoglutaramate + NH3 + NADH | - |
? | |
L-glutamine + H2O + NAD+ | - |
Citrobacter sp. | 2-oxoglutaramate + NH3 + NADH | - |
? | |
L-glutamine + H2O + NAD+ | - |
Proteus sp. | 2-oxoglutaramate + NH3 + NADH | - |
? | |
L-isoleucine + H2O + NAD+ | best substrate | Citrobacter sp. | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
? | |
L-methionine + H2O + NAD+ | - |
Proteus sp. | (4-methylsulfanyl)-2-oxobutanoate + NH3 + NADH | - |
? | |
L-serine + H2O + NAD+ | - |
Pseudomonas sp. | 3-hydroxy-2-oxopropanoate + NH3 + NADH | - |
? | |
L-serine + H2O + NAD+ | - |
Citrobacter sp. | 3-hydroxy-2-oxopropanoate + NH3 + NADH | - |
? | |
L-serine + H2O + NAD+ | - |
Proteus sp. | 3-hydroxy-2-oxopropanoate + NH3 + NADH | - |
? | |
L-valine + H2O + NAD+ | highest activity | Pseudomonas sp. | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? | |
additional information | no amino acid dehydrogenase activity for L-asparagine | Proteus sp. | ? | - |
? | |
additional information | no amino acid dehydrogenase activity for L-isoleucine amino acid analogs including L-leucine, L-valine, L-alanine or L-methionine | Citrobacter sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAD+-dependent amino acid dehydrogenase | - |
Pseudomonas sp. |
NAD+-dependent amino acid dehydrogenase | - |
Citrobacter sp. |
NAD+-dependent amino acid dehydrogenase | - |
Proteus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.15 | - |
L-glutamine | in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Proteus sp. | |
2.11 | - |
L-methionine | in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Proteus sp. | |
2.84 | - |
L-isoleucine | in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Citrobacter sp. | |
4.95 | - |
L-valine | in 100 mM glycine-KCl-KOH buffer (pH 10.4) | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent | Pseudomonas sp. | |
NAD+ | dependent | Citrobacter sp. | |
NAD+ | dependent | Proteus sp. |