Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of enzyme deletion mutants DELTAgdh1 and DELTAgdh3, the mutants show less than 20% of wild-type activity, genotypes and phenotypes, overview | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH3 + NADPH + H+ | Saccharomyces cerevisiae | - |
L-glutamate + H2O + NADP+ | - |
ir | |
additional information | Saccharomyces cerevisiae | in yeast, NADP+-dependent enzymes, EC 1.4.1.4, encoded by GDH1 and GDH3, are reported to synthesize glutamate from 2-oxtoglutarate, while an NAD+-dependent enzyme, EC 1.4.1.2, encoded by GDH2, catalyzes the reverse reaction. Gdh1p is the primary GDH enzyme and Gdh2p and Gdh3p play evident roles during aerobic glutamate metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:acetate/raffinose-grown cell | - |
Saccharomyces cerevisiae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH3 + NADPH + H+ | - |
Saccharomyces cerevisiae | L-glutamate + H2O + NADP+ | - |
ir | |
additional information | in yeast, NADP+-dependent enzymes, EC 1.4.1.4, encoded by GDH1 and GDH3, are reported to synthesize glutamate from 2-oxtoglutarate, while an NAD+-dependent enzyme, EC 1.4.1.2, encoded by GDH2, catalyzes the reverse reaction. Gdh1p is the primary GDH enzyme and Gdh2p and Gdh3p play evident roles during aerobic glutamate metabolism | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GDH3 | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
physiological function | Gdh1p is the primary GDH enzyme and Gdh3p plays an evident role during aerobic glutamate metabolism | Saccharomyces cerevisiae |