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Literature summary for 1.4.1.4 extracted from

  • Tang, Y.; Sieg, A.; Trotter, P.J.
    13C-metabolic enrichment of glutamate in glutamate dehydrogenase mutants of Saccharomyces cerevisiae (2011), Microbiol. Res., 166, 521-530.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information construction of enzyme deletion mutants DELTAgdh1 and DELTAgdh3, the mutants show less than 20% of wild-type activity, genotypes and phenotypes, overview Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxoglutarate + NH3 + NADPH + H+ Saccharomyces cerevisiae
-
L-glutamate + H2O + NADP+
-
ir
additional information Saccharomyces cerevisiae in yeast, NADP+-dependent enzymes, EC 1.4.1.4, encoded by GDH1 and GDH3, are reported to synthesize glutamate from 2-oxtoglutarate, while an NAD+-dependent enzyme, EC 1.4.1.2, encoded by GDH2, catalyzes the reverse reaction. Gdh1p is the primary GDH enzyme and Gdh2p and Gdh3p play evident roles during aerobic glutamate metabolism ?
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?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:acetate/raffinose-grown cell
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Saccharomyces cerevisiae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADPH + H+
-
Saccharomyces cerevisiae L-glutamate + H2O + NADP+
-
ir
additional information in yeast, NADP+-dependent enzymes, EC 1.4.1.4, encoded by GDH1 and GDH3, are reported to synthesize glutamate from 2-oxtoglutarate, while an NAD+-dependent enzyme, EC 1.4.1.2, encoded by GDH2, catalyzes the reverse reaction. Gdh1p is the primary GDH enzyme and Gdh2p and Gdh3p play evident roles during aerobic glutamate metabolism Saccharomyces cerevisiae ?
-
?

Synonyms

Synonyms Comment Organism
GDH3
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Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
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assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Saccharomyces cerevisiae

General Information

General Information Comment Organism
physiological function Gdh1p is the primary GDH enzyme and Gdh3p plays an evident role during aerobic glutamate metabolism Saccharomyces cerevisiae