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Literature summary for 1.4.1.4 extracted from

  • Choudhury, R.; Punekar, N.S.
    Aspergillus terreus NADP-glutamate dehydrogenase is kinetically distinct from the allosteric enzyme of other Aspergilli (2009), Mycol. Res., 113, 1121-1126.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ATP 30% stimulation in the presence of 2 mM Mg2+ Aspergillus terreus
GDP 30% stimulation in the presence of 2 mM Mg2+ Aspergillus terreus
GTP 30% stimulation in the presence of 2 mM Mg2+ Aspergillus terreus

General Stability

General Stability Organism
after incubation with chymotrypsin enzyme loses 50% activity within 30 min and a combination of 2-oxoglutarate and NADP+ affords near complete protection against chymotrypsin Aspergillus niger
Aspergillus terreus NADP-GDH remains fully active even after 2 h of incubation with chymotrypsin Aspergillus terreus

Inhibitors

Inhibitors Comment Organism Structure
2,4-pyridinedicarboxylate weak inhibitor Aspergillus terreus
2-Methyleneglutarate weak inhibitor Aspergillus terreus
isophthalate weak inhibitor Aspergillus terreus
NH4+ inhibits the oxidative deamination activity as a product, inhibition is non-competitive with respect to L-glutamate Aspergillus terreus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
NADPH 28°C Aspergillus niger
0.017
-
NADP+ 28°C Aspergillus niger
0.045
-
NADP+ 28°C Aspergillus terreus
0.055
-
NADPH 28°C Aspergillus terreus
0.95
-
NH4+ 28°C Aspergillus terreus
1.05
-
NH4+ 28°C Aspergillus niger
4.78
-
2-oxoglutarate 28°C Aspergillus niger
6
-
2-oxoglutarate 28°C Aspergillus terreus
24.1
-
L-glutamate 28°C Aspergillus terreus
34.6
-
L-glutamate 28°C Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Aspergillus terreus
Zn2+ forward reaction of GDH from Aspergillus terreus is 80% inhibited by 5 mM ZnCl2 Aspergillus terreus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
SDS-PAGE Aspergillus terreus
350000
-
FPLC on a Superdex G-75 column Aspergillus terreus

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
GDH from Aspergillus terreus is kinetically distinct from GDH of Aspergilli niger
-
Aspergillus terreus
-
GDH from Aspergillus terreus is kinetically distinct from GDH of Aspergilli niger
-

Purification (Commentary)

Purification (Comment) Organism
purified to homogeneity using novel reactive dye-affinity resins Aspergillus niger
purified to homogeneity using novel reactive dye-affinity resins Aspergillus terreus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH4+ + NADPH
-
Aspergillus niger L-glutamate + NADP+
-
?
2-oxoglutarate + NH4+ + NADPH
-
Aspergillus terreus L-glutamate + NADP+
-
?

Subunits

Subunits Comment Organism
hexamer
-
Aspergillus niger
hexamer 6 x 50000, SDS-PAGE Aspergillus terreus

Synonyms

Synonyms Comment Organism
NADP-glutamate dehydrogenase
-
Aspergillus niger
NADP-glutamate dehydrogenase
-
Aspergillus terreus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Aspergillus niger
28
-
assay at Aspergillus terreus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
reductive amination Aspergillus terreus
9.5
-
oxidative deamination Aspergillus terreus

Cofactor

Cofactor Comment Organism Structure
NADPH specifically used Aspergillus niger
NADPH specifically used Aspergillus terreus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.17
-
NH4+
-
Aspergillus terreus