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Literature summary for 1.4.1.4 extracted from

  • Ha, J.S.; Kim, K.; Song, J.J.; Bae, J.W.; Lee, S.G.; Lee, S.C.; Poo, H.; Shin, C.S.; Sung, M.H.
    Thermostable glutamate dehydrogenase from a commensal thermophile,Symbiobacterium toebii; overproduction, characterization, and application (2003), J. Mol. Catal. B, 26, 231-240.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli DH5alpha under the control of a highly constitutive expresion promoter in a pHCE system Symbiobacterium toebii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.088
-
NADP+ pH 9.0, 50°C Symbiobacterium toebii
0.28
-
NADPH pH 9.0, 50°C Symbiobacterium toebii
0.368
-
NADH pH 9.0, 50°C Symbiobacterium toebii
3.629
-
NAD+ pH 9.0, 50°C Symbiobacterium toebii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
6 * 44000, SDS-PAGE Symbiobacterium toebii
263000
-
gel filtration Symbiobacterium toebii

Organism

Organism UniProt Comment Textmining
Symbiobacterium toebii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Symbiobacterium toebii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
32.8
-
pH 9.0, 50°C Symbiobacterium toebii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH
-
Symbiobacterium toebii L-glutamate + H2O + NAD+
-
r
2-oxoglutarate + NH3 + NADPH
-
Symbiobacterium toebii L-glutamate + H2O + NADP+
-
r

Subunits

Subunits Comment Organism
hexamer 6 * 44000, SDS-PAGE Symbiobacterium toebii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Symbiobacterium toebii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 70 50°C: about 65% of maximal activity, 70°C: about 85% of maximal activity Symbiobacterium toebii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
20 h, stable Symbiobacterium toebii
40 55 30 min, stable Symbiobacterium toebii
80
-
30 min, complete loss of activity Symbiobacterium toebii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
NAD+ pH 9.0, 50°C Symbiobacterium toebii
2136
-
NADP+ pH 9.0, 50°C Symbiobacterium toebii
6144
-
NADH pH 9.0, 50°C Symbiobacterium toebii
28650
-
NADPH pH 9.0, 50°C Symbiobacterium toebii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Symbiobacterium toebii

pH Range

pH Minimum pH Maximum Comment Organism
8.5 10 pH 8.5: about 60% of maximal activity, pH 10.0: about 40% of maximal activity Symbiobacterium toebii

Cofactor

Cofactor Comment Organism Structure
NAD+ the ratio of turnover-number to KM-value for NADP+ is 72679fold higher than the ratio for NAD+ Symbiobacterium toebii
NADH the ratio of turnover-number to KM-value for NADPH is 6fold higher than the ratio for NADH Symbiobacterium toebii
NADP+ preferred coenzyme. The ratio of turnover-number to KM-value for NADP+ is 72679fold higher than the ratio for NAD+ Symbiobacterium toebii
NADPH preferred coenzyme. The ratio of turnover-number to KM-value for NADPH is 6fold higher than the ratio for NADH Symbiobacterium toebii