Any feedback?
Literature summary for 1.4.1.4 extracted from
- Purification and some properties of the NADP-linked glutamate dehydrogenase from Trypanosoma cruzi (1978), Int. J. Biochem., 9, 395-400.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| KCl | up to 50mM | Trypanosoma cruzi |  |
| NaCl | up to 50 mM | Trypanosoma cruzi | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCl | more than 50 mM | Trypanosoma cruzi | |
| NaCl | more than 50 mM | Trypanosoma cruzi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
NADPH | reductive amination | Trypanosoma cruzi | |
| 0.0064 | - |
NADP+ | oxidative deamination | Trypanosoma cruzi | |
| 1 | - |
2-oxoglutarate | reductive amination | Trypanosoma cruzi | |
| 1.7 | - |
NH4+ | reductive amination | Trypanosoma cruzi | |
| 2 | - |
L-glutamate | reductive amination | Trypanosoma cruzi | |
| 66 | - |
NH4+ | reductive amination | Trypanosoma cruzi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
4 * 64000, SDS-PAGE | Trypanosoma cruzi |
| 280000 | - |
gel filtration | Trypanosoma cruzi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NADP+ + H2O | Trypanosoma cruzi | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma cruzi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trypanosoma cruzi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 51.2 | - |
reductive amination | Trypanosoma cruzi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + NH4+ + NADPH | - |
Trypanosoma cruzi | L-glutamate + NADP+ | - |
? | |
| L-glutamate + NADP+ + H2O | - |
Trypanosoma cruzi | 2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | - |
Trypanosoma cruzi | 2-oxoglutarate + NADPH + NH3 | biphasic kinetic behavior for ammonia | r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 64000, SDS-PAGE | Trypanosoma cruzi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
oxidative deamination | Trypanosoma cruzi |
| 8 | - |
reductive amination | Trypanosoma cruzi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Trypanosoma cruzi | |
| NADPH | - |
Trypanosoma cruzi | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
