Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | at 0.3 mM 40% activation | Proteus inconstans | |
ATP | at 0.3 mM, 60% activation of reductive amination | Proteus inconstans |
Crystallization (Comment) | Organism |
---|---|
dialysis against ammonium sulfate solution | Proteus inconstans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | at 0.3 mM, 22% inhibition of oxidative deamination | Proteus inconstans | |
AMP | at 0.3 mM 33% inhibition of oxidative deamination | Proteus inconstans | |
Hg2+ | at 1 mM, 100% activity loss | Proteus inconstans | |
iodoacetamide | at 4 mM, complete inactivation | Proteus inconstans | |
N-ethylmaleimide | at 0.8 mM, 44% inhibition | Proteus inconstans | |
p-chloromercuriphenyl sulfonate | inactivetes, can be reversed by addition of cysteine | Proteus inconstans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
NADPH | reductive amination | Proteus inconstans | |
0.023 | - |
NADP+ | oxidative deamination | Proteus inconstans | |
0.37 | - |
NH4+ | reductive amination | Proteus inconstans | |
2.3 | - |
L-glutamate | oxidative deamination | Proteus inconstans | |
3.7 | - |
L-glutamate | reductive amination | Proteus inconstans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Proteus inconstans |
400000 | - |
gel filtration | Proteus inconstans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Proteus inconstans | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Proteus inconstans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Proteus inconstans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
231 | - |
oxidative deamination at 25°C | Proteus inconstans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH4+ + NADPH | - |
Proteus inconstans | L-glutamate + NADP+ | - |
? | |
L-glutamate + NADP+ + H2O | - |
Proteus inconstans | 2-oxoglutarate + NADPH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Proteus inconstans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Proteus inconstans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
irreversible heat inactivation | Proteus inconstans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
reductive amination | Proteus inconstans |
10 | - |
oxidative deamination | Proteus inconstans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | unstable below pH 5.0 or above pH 10.0 | Proteus inconstans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Proteus inconstans | |
NADPH | - |
Proteus inconstans |