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Literature summary for 1.4.1.4 extracted from

  • Shimizu, H.; Kuratsu, T.; Hirata, F.
    Purification and some properties of glutamate dehydrogenase from Proteus inconstans (1979), J. Ferment. Technol., 57, 428-433.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
ADP at 0.3 mM 40% activation Proteus inconstans
ATP at 0.3 mM, 60% activation of reductive amination Proteus inconstans

Crystallization (Commentary)

Crystallization (Comment) Organism
dialysis against ammonium sulfate solution Proteus inconstans

Inhibitors

Inhibitors Comment Organism Structure
ADP at 0.3 mM, 22% inhibition of oxidative deamination Proteus inconstans
AMP at 0.3 mM 33% inhibition of oxidative deamination Proteus inconstans
Hg2+ at 1 mM, 100% activity loss Proteus inconstans
iodoacetamide at 4 mM, complete inactivation Proteus inconstans
N-ethylmaleimide at 0.8 mM, 44% inhibition Proteus inconstans
p-chloromercuriphenyl sulfonate inactivetes, can be reversed by addition of cysteine Proteus inconstans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
NADPH reductive amination Proteus inconstans
0.023
-
NADP+ oxidative deamination Proteus inconstans
0.37
-
NH4+ reductive amination Proteus inconstans
2.3
-
L-glutamate oxidative deamination Proteus inconstans
3.7
-
L-glutamate reductive amination Proteus inconstans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE Proteus inconstans
400000
-
gel filtration Proteus inconstans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + NADP+ + H2O Proteus inconstans
-
2-oxoglutarate + NADPH + NH3
-
r

Organism

Organism UniProt Comment Textmining
Proteus inconstans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Proteus inconstans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
231
-
oxidative deamination at 25°C Proteus inconstans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH4+ + NADPH
-
Proteus inconstans L-glutamate + NADP+
-
?
L-glutamate + NADP+ + H2O
-
Proteus inconstans 2-oxoglutarate + NADPH + NH3
-
r

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Proteus inconstans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Proteus inconstans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
irreversible heat inactivation Proteus inconstans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
reductive amination Proteus inconstans
10
-
oxidative deamination Proteus inconstans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 unstable below pH 5.0 or above pH 10.0 Proteus inconstans

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Proteus inconstans
NADPH
-
Proteus inconstans