Literature summary for 1.4.1.4 extracted from

Martinez-Bilbao, M.; Martinez, A.; Urkijo, I.; Llama, M.J.; Serra, J.L.
Induction, isolation, and some properties of the NADPH-dependent glutamate dehydrogenase from the nonheterocystous cyanobacterium Phormidium laminosum (1988), J. Bacteriol., 170, 4897-4902.

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General Stability

General Stability	Organism
unstable when frozen at -20°C after a few h even with addition of 50% v/v glycerol	Phormidium laminosum

Inhibitors

Inhibitors	Comment	Organism	Structure
Glutaric acid	at 20 mM, 25% inhibition	Phormidium laminosum
L-Glutamic acid	at 20 mM 25% inhibition	Phormidium laminosum
L-tryptophan	at 20 mM, 15% inhibition	Phormidium laminosum
Urea	no activity at 6 mM	Phormidium laminosum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.064	-	NADPH	reductive amination	Phormidium laminosum
1.25	-	2-oxoglutarate	reductive amination	Phormidium laminosum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
280000	-	gel filtration	Phormidium laminosum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-glutamate + NADP+ + H2O	Phormidium laminosum	-	2-oxoglutarate + NADPH + NH3	-	r

Organism

Organism	UniProt	Comment	Textmining
Phormidium laminosum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Phormidium laminosum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
32.9	-	reductive amination at 30°C	Phormidium laminosum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamate + NADP+ + H2O	-	Phormidium laminosum	2-oxoglutarate + NADPH + NH3	-	r

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	reductive amination	Phormidium laminosum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
72	-	complete inactivation	Phormidium laminosum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	reductive amination in potassium phosphate buffer	Phormidium laminosum
8	-	reductive amination in Tris hydrochloride buffer	Phormidium laminosum

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Phormidium laminosum
NADPH	-	Phormidium laminosum

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Release 2023.1 (January 2023)