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Literature summary for 1.4.1.4 extracted from
- Purification and characterization of the NADP-dependent glutamate dehydrogenase from Bacillus fastidiosus (1989), Antonie van Leeuwenhoek, 55, 303-311.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fumarate | at 5 mM 20% inhibition | Metabacillus fastidiosus |  |
| malate | at 5 mM, 20% inhibition | Metabacillus fastidiosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
NADP+ | oxidative deamination | Metabacillus fastidiosus | |
| 0.027 | - |
NADPH | reductive amination | Metabacillus fastidiosus | |
| 0.5 | - |
2-oxoglutarate | reductive amination | Metabacillus fastidiosus | |
| 6.5 | - |
NH4+ | reductive amination | Metabacillus fastidiosus | |
| 20 | - |
NH4+ | reductive amination | Metabacillus fastidiosus | |
| 44 | - |
L-glutamate | oxidative deamination | Metabacillus fastidiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
alpha6, 6 * 48000, SDS-PAGE | Metabacillus fastidiosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NADP+ + H2O | Metabacillus fastidiosus | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Metabacillus fastidiosus | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 388 | - |
reductive amination | Metabacillus fastidiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + NH4+ + NADPH | - |
Metabacillus fastidiosus | L-glutamate + NADP+ | - |
? | |
| L-glutamate + NADP+ + H2O | - |
Metabacillus fastidiosus | 2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | - |
Metabacillus fastidiosus | 2-oxoglutarate + NADPH + NH3 | biphasic kinetic behavior for ammonia | r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | alpha6, 6 * 48000, SDS-PAGE | Metabacillus fastidiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Metabacillus fastidiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
9% activity loss after 5 min | Metabacillus fastidiosus |
| 70 | - |
complete inactivation | Metabacillus fastidiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
reductive amination | Metabacillus fastidiosus |
| 8.6 | - |
oxidative deamination | Metabacillus fastidiosus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Metabacillus fastidiosus | |
| NADPH | - |
Metabacillus fastidiosus | |
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