Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | at 5 mM, 135% activation, oxidative deamination | Thermococcus sp. | |
MgCl2 | at 5 mM, 104% activity, oxidative deamination | Thermococcus sp. | |
MnCl2 | at 5 mM, 250% activity, oxidative deamination | Thermococcus sp. |
General Stability | Organism |
---|---|
10 mM MOPS-NaOH pH 7.1 and 0.3-0.4 M NaCl is the best buffer | Thermococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | at 1 mM, no activity, oxidative deamination | Thermococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
NADP+ | oxidative deamination at 80°C | Thermococcus sp. | |
0.066 | - |
NADPH | reductive amination at 80°C | Thermococcus sp. | |
1.7 | - |
2-oxoglutarate | reductive amination at 80°C | Thermococcus sp. | |
9.12 | - |
L-glutamate | oxidative deamination at 80°C | Thermococcus sp. | |
15.5 | - |
NH4+ | reductive amination at 80°C | Thermococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
alpha4, 4 * 47000, SDS-PAGE | Thermococcus sp. |
204000 | - |
gel filtration | Thermococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Thermococcus sp. | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus sp. | - |
isolate AN1 | - |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
464 | - |
oxidative deamination at 80°C and pH 7.54 | Thermococcus sp. |
2940 | - |
reductive amination at 80°C and pH 7.54 | Thermococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH4+ + NADPH | - |
Thermococcus sp. | L-glutamate + NADP+ | - |
? | |
L-glutamate + NADP+ + H2O | - |
Thermococcus sp. | 2-oxoglutarate + NADPH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | alpha4, 4 * 47000, SDS-PAGE | Thermococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
50% activity after 12.5 h in 0.1 M NaH2PO4/Na2HPO4 | Thermococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
reductive amination and oxidative deamination | Thermococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Thermococcus sp. | |
NADPH | - |
Thermococcus sp. |