Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCl | optimal activity at 1-1.5 M | Haloferax mediterranei | |
NaCl | optimal activity at 1-1.5 M | Haloferax mediterranei |
General Stability | Organism |
---|---|
in the presence of 4 M NaCl the purified enzyme remains fully active at room temperature for months. The stability is rapidly lost upon lowering the salt concentration | Haloferax mediterranei |
NaCl and KCl markedly increase the thermostability | Haloferax mediterranei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
NADPH | reductive amination at 25°C | Haloferax mediterranei | |
0.018 | - |
2-oxoglutarate | pH 8.5, 40°C | Haloferax mediterranei | |
0.34 | - |
2-oxoglutarate | reductive amination at 25°C | Haloferax mediterranei | |
0.34 | - |
NH3 | pH 8.5, 40°C | Haloferax mediterranei | |
4.2 | - |
NH4+ | reductive amination at 25°C | Haloferax mediterranei | |
4.2 | - |
NADPH | pH 8.5, 40°C | Haloferax mediterranei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | stimulating effect of KCl is slightly higher than the effect of identical concentrations of NaCl | Haloferax mediterranei | |
NaCl | stimulating effect of KCl is slightly higher than the effect of identical concentrations of NaCl | Haloferax mediterranei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
alpha6, 6 * 48000, CTAB-PAGE | Haloferax mediterranei |
55000 | - |
alpha6, 6 * 55000, SDS-PAGE | Haloferax mediterranei |
55000 | - |
6 * 55000, SDS-PAGE | Haloferax mediterranei |
320000 | - |
gel filtration | Haloferax mediterranei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Haloferax mediterranei | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | Haloferax mediterranei DSM 1411 | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organic Solvent | Comment | Organism |
---|---|---|
Glycerol | stabilized by glycerol in absence of salt | Haloferax mediterranei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax mediterranei | - |
- |
- |
Haloferax mediterranei DSM 1411 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloferax mediterranei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
60 | - |
reductive amination at 40°C | Haloferax mediterranei |
60 | - |
pH 8.5, 40°C | Haloferax mediterranei |
Storage Stability | Organism |
---|---|
25°C, 20% v/v glycerol, 476 h, 50% activity | Haloferax mediterranei |
fully active at 4 M concentration NaCl at room temperature for months | Haloferax mediterranei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH3 + NADPH + H+ | - |
Haloferax mediterranei | L-glutamate + H2O + NADP+ | - |
? | |
2-oxoglutarate + NH3 + NADPH + H+ | - |
Haloferax mediterranei DSM 1411 | L-glutamate + H2O + NADP+ | - |
? | |
2-oxoglutarate + NH4+ + NADPH | - |
Haloferax mediterranei | L-glutamate + NADP+ | - |
? | |
2-oxoglutarate + NH4+ + NADPH | - |
Haloferax mediterranei DSM 1411 | L-glutamate + NADP+ | - |
? | |
L-glutamate + NADP+ + H2O | - |
Haloferax mediterranei | 2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | - |
Haloferax mediterranei DSM 1411 | 2-oxoglutarate + NADPH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 55000, SDS-PAGE | Haloferax mediterranei |
homohexamer | alpha6, 6 * 55000, SDS-PAGE | Haloferax mediterranei |
homohexamer | alpha6, 6 * 48000, CTAB-PAGE | Haloferax mediterranei |
Synonyms | Comment | Organism |
---|---|---|
NADP(H)-specific glutamate dehydrogenase | - |
Haloferax mediterranei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Haloferax mediterranei |
60 | - |
- |
Haloferax mediterranei |
60 | - |
reductive amination | Haloferax mediterranei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the thermostability decreases significantly as the salt concentration is lowered | Haloferax mediterranei |
67 | - |
t1/2 in presence of 2 M KCl is about 27 min, while in 4 M KCl the t1/2 is 70 h | Haloferax mediterranei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Haloferax mediterranei |
8.5 | - |
reductive amination | Haloferax mediterranei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Haloferax mediterranei | |
NADPH | - |
Haloferax mediterranei |