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Literature summary for 1.4.1.4 extracted from
- An NADP-glutamate dehydrogenase from the green alga Bryopsis maxima. Purification and properties (1997), Plant Cell Physiol., 38, 327-335.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | at 1 mM, 100% activity loss | Bryopsis maxima |  |
| Mg2+ | at 1 mM, 16% activity loss | Bryopsis maxima | |
| Mn2+ | at 1 mM, 19% activity loss | Bryopsis maxima | |
| Zn2+ | at 1 mM, 64% activity loss | Bryopsis maxima | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
NADP+ | oxidative deamination at 25°C | Bryopsis maxima | |
| 0.03 | - |
NADPH | reductive amination at 25°C | Bryopsis maxima | |
| 2.2 | - |
NH4+ | reductive amination at 25°C | Bryopsis maxima | |
| 3 | - |
2-oxoglutarate | reductive amination at 25°C | Bryopsis maxima | |
| 3.2 | - |
L-glutamate | oxidative deamination at 25°C | Bryopsis maxima | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
alpha6, 6 * 46000, SDS-PAGE | Bryopsis maxima |
| 280000 | - |
gel filtration | Bryopsis maxima |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NADP+ + H2O | Bryopsis maxima | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bryopsis maxima | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bryopsis maxima |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 18.4 | - |
oxidative deamination at 25°C | Bryopsis maxima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + NH4+ + NADPH | - |
Bryopsis maxima | L-glutamate + NADP+ | - |
? | |
| L-glutamate + NADP+ + H2O | - |
Bryopsis maxima | 2-oxoglutarate + NADPH + NH3 | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | alpha6, 6 * 46000, SDS-PAGE | Bryopsis maxima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
maximal temperature for activity, 60 min stable | Bryopsis maxima |
| 50 | - |
10 min stable | Bryopsis maxima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
reductive amination | Bryopsis maxima |
| 8.6 | - |
oxidative deamination | Bryopsis maxima |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 8 | - |
Bryopsis maxima |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Bryopsis maxima | |
| NADPH | - |
Bryopsis maxima | |
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Release 2023.1 (January 2023)
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