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Literature summary for 1.4.1.4 extracted from

  • Hatanaka, M.; Tachiki, T.; Kumagai, H.; Tochikura, T.
    Distribution and some properties of glutamine synthetase and glutamate dehydrogenase in Bifidobacteria (1987), Agric. Biol. Chem., 51, 251-252.
No PubMed abstract available
Search for more literature for 1.4.1.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.095
-
 NADPH reductive amination Bifidobacterium bifidum
0.77
-
 2-oxoglutarate reductive amination Bifidobacterium bifidum
2
-
 NH4+ reductive amination Bifidobacterium bifidum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + NADP+ + H2O Bifidobacterium bifidum
-
 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O Bifidobacterium adolescentis
-
 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O Bifidobacterium breve
-
 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O Bifidobacterium pseudolongum
-
 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O Bifidobacterium longum subsp. infantis
-
 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O Bifidobacterium thermophilum
-
 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O Bifidobacterium longum
-
 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O Bifidobacterium bifidum A
-
 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O Bifidobacterium thermophilum B
-
 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O Bifidobacterium pseudolongum A
-
 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O Bifidobacterium pseudolongum B
-
 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O Bifidobacterium bifidum B
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

Organism UniProt Comment Textmining
Bifidobacterium adolescentis
-
-
-
Bifidobacterium bifidum
-
 hosts human adult and human infant
-
Bifidobacterium breve
-
 strain breve a and parvolorum a
-
Bifidobacterium longum
-
 hosts rat and calf
-
Bifidobacterium longum subsp. infantis
-
 strain infantis a and liberorum
-
Bifidobacterium pseudolongum
-
 the hosts are pig and chicken
-
Bifidobacterium thermophilum
-
 host pig
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.29
-
 host human adult, reductive amination at 30°C and pH 6.5 Bifidobacterium bifidum
0.31
-
 host human infant, reductive amination at 30°C and pH 6.5 Bifidobacterium bifidum
0.34
-
 host chicken, reductive amination at 30°C and pH 6.5 Bifidobacterium pseudolongum
0.4
-
 reductive amination at 30°C and pH 6.5 Bifidobacterium thermophilum
0.45
-
 host calf, reductive amination at 30°C and pH 6.5 Bifidobacterium longum
0.59
-
 host rat, reductive amination at 30°C and pH 6.5 Bifidobacterium longum
0.62
-
 strain parvolorum, reductive amination at 30°C and pH 6.5 Bifidobacterium breve
0.7
-
 reductive amination at 30°C and pH 6.5 Bifidobacterium adolescentis
0.7
-
 reductive amination at 30°C and pH 6.5 Bifidobacterium pseudolongum
0.7
-
 host pig Bifidobacterium pseudolongum
0.8
-
 strain liberorum, reductive amination at 30°C and pH 6.5 Bifidobacterium longum subsp. infantis
1.1
-
 strain breve a, reductive amination at 30°C and pH 6.5 Bifidobacterium breve
1.16
-
 strain infantis a, reductive amination at 30°C and pH 6.5 Bifidobacterium longum subsp. infantis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH4+ + NADPH
-
 Bifidobacterium bifidum L-glutamate + NADP+
-
 ?
2-oxoglutarate + NH4+ + NADPH
-
 Bifidobacterium bifidum A L-glutamate + NADP+
-
 ?
2-oxoglutarate + NH4+ + NADPH
-
 Bifidobacterium bifidum B L-glutamate + NADP+
-
 ?
L-glutamate + NADP+ + H2O
-
 Bifidobacterium bifidum 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O
-
 Bifidobacterium adolescentis 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O
-
 Bifidobacterium breve 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O
-
 Bifidobacterium pseudolongum 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O
-
 Bifidobacterium longum subsp. infantis 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O
-
 Bifidobacterium thermophilum 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O
-
 Bifidobacterium longum 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O
-
 Bifidobacterium bifidum A 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O
-
 Bifidobacterium thermophilum B 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O
-
 Bifidobacterium pseudolongum A 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O
-
 Bifidobacterium pseudolongum B 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O
-
 Bifidobacterium bifidum B 2-oxoglutarate + NADPH + NH3
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 reductive amination Bifidobacterium bifidum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Bifidobacterium bifidum
NADP+
-
 Bifidobacterium adolescentis
NADP+
-
 Bifidobacterium breve
NADP+
-
 Bifidobacterium pseudolongum
NADP+
-
 Bifidobacterium longum subsp. infantis
NADP+
-
 Bifidobacterium thermophilum
NADP+
-
 Bifidobacterium longum
NADPH
-
 Bifidobacterium bifidum
NADPH
-
 Bifidobacterium adolescentis
NADPH
-
 Bifidobacterium breve
NADPH
-
 Bifidobacterium pseudolongum
NADPH
-
 Bifidobacterium longum subsp. infantis
NADPH
-
 Bifidobacterium thermophilum
NADPH
-
 Bifidobacterium longum