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Literature summary for 1.4.1.3 extracted from

  • Zaganas, I.; Kanavouras, K.; Mastorodemos, V.; Latsoudis, H.; Spanaki, C.; Plaitakis, A.
    The human GLUD2 glutamate dehydrogenase: localization and functional aspects (2009), Neurochem. Int., 55, 52-63.
    View publication on PubMed
Search for more literature for 1.4.1.3

Activating Compound

Activating Compound Comment Organism Structure
ADP ADP displays a lower affinity for hGDH2 than for hGDH1 Homo sapiens
L-leucine L-leucine displays the same affinity for hGDH2 and hGDH1 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf21 cells using the baculovirus expression system Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
GTP efective inhibitor: activity below 10% of its full activity; GDH2 is resistent to GTP inhibition Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Homo sapiens 5783
-
mitochondrion
-
 Homo sapiens 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 native gradient polyacryamide gel electrophoresis, 6 * 60000 Da Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulphate fractionation, hydrophobic interaction (using a phenylsepharose high performance column) and hydroxyapatite chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 hGDH1 displays a high basal activity of 40% of its maximal activity. Regulation of the hGDH1 activity is achieved by interplay of GTP and ADP/L-leucine Homo sapiens
additional information
-
 hGDH2 displays a basal activity less than 10% of its maximal activity. Control of the hGDH2 activity depends on the level of its basal activity and on changing ADP/L-leucine concentrations. For hGDH2 enzyme protein concentration and basal activity show a linear pattern of dependence. Lowering the temperature of the reaction mixture from 25°C to 20°C has a stabilizing effect on hGDH2, as under these conditions, the specific basal activities of the enzyme increases. Increasing the concentration of the Tris–HCl buffer from 50 mM to 125 mM or to 250 mM (pH 8.0) raises basal activity levels, but the maximal activity declines Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NAD(P)H + NH3
-
 Homo sapiens L-glutamate + NAD(P)+ + H2O
-
 ?

Subunits

Subunits Comment Organism
hexamer native gradient polyacryamide gel electrophoresis, 6 * 60000 Da Homo sapiens

Synonyms

Synonyms Comment Organism
glutamate dehydrogenase 1
-
 Homo sapiens
glutamate dehydrogenase 2
-
 Homo sapiens
hGLUD1
-
 Homo sapiens
hGLUD2
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 in TRA buffer hGDH2 displays its maximal activity and its highest basal activity at pH 7.5 Homo sapiens
7.8
-
 highest basal activity in phosphate buffer Homo sapiens
8
-
 assay at Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7.75 8 maximal specific activity in TRA buffer Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00031
-
 in the absence of other modulators, pH 8.0 Homo sapiens GTP
0.00161
-
 in the presence of 0.1 mM ADP, pH 8.0 Homo sapiens GTP
0.0185
-
 in the presence of 1 mM ADP, pH 8.0 Homo sapiens GTP
0.0239
-
 in the presence of 0.1 mM ADP, pH 8.0 Homo sapiens GTP
0.0785
-
 in the absence of other modulators, pH 8.0 Homo sapiens GTP
0.1668
-
 in the presence of 1 mM ADP, pH 8.0 Homo sapiens GTP