Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | activates at 1 mM | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in sf21 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R443S/G456A | resistant to GTP inhibition | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GTP | inhibits isozyme GDH1 | Homo sapiens | |
additional information | GDH2 is resistant to GTP inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
2-oxoglutarate | mutant enzyme R443S/G456A, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP | Homo sapiens | |
2 | - |
2-oxoglutarate | wild type isozyme GDH1, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP | Homo sapiens | |
2.1 | - |
2-oxoglutarate | wild type isozyme GDH2, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP | Homo sapiens | |
10.7 | - |
L-glutamate | wild type isozyme GDH2, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP | Homo sapiens | |
10.8 | - |
L-glutamate | mutant enzyme R443S/G456A, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP | Homo sapiens | |
12.4 | - |
L-glutamate | wild type isozyme GDH1, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP | Homo sapiens | |
13.4 | - |
NH3 | wild type isozyme GDH1, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP | Homo sapiens | |
17.1 | - |
NH3 | wild type isozyme GDH2, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP | Homo sapiens | |
22.2 | - |
NH3 | mutant enzyme R443S/G456A, in 50 mM triethanolamine buffer (pH 8.0), 2.6 mM EDTA, 1.4 mM NADP+, and 1 mM ADP | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
6 * 56000, SDS-PAGE | Homo sapiens |
336000 | - |
SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, phenyl-Sepharose column chromatography, and hydroxyapatite chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
nerve | - |
Homo sapiens | - |
testis | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
145.7 | - |
purified mutant enzyme R443S/G456A | Homo sapiens |
159.1 | - |
purified isozyme GDH2 | Homo sapiens |
161.9 | - |
purified isozyme GDH1 | Homo sapiens |
Storage Stability | Organism |
---|---|
5°C, sodium phosphate buffer pH 6.8, several months, no loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD(P)+ | - |
Homo sapiens | 2-oxoglutarate + NH3 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 56000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
GDH1 | isozyme | Homo sapiens |
GDH2 | isozyme | Homo sapiens |
GLUD2 | isozyme | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
47.5 | - |
GDH2 has a half-life of 38 min at 47.5°C, GDH1 has a half-life of 348 min at 47.5°C, high concentrations of phosphate (300 mM) have a protective effect on the thermal denaturation of both wild type hGDHs, this effect being more pronounced for GDH2 | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
isozyme GDH2 | Homo sapiens |
7.5 | 8 | isozyme GDH1 | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)+ | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.31 | - |
GDH1, in the absence of ADP, in 50 mM triethanolamine pH 8.0 buffer | Homo sapiens | GTP | |
18.5 | - |
GDH1, in the presence of 1 mM ADP, in 50 mM triethanolamine pH 8.0 buffer | Homo sapiens | GTP | |
78.5 | - |
GDH2, in the absence of ADP, in 50 mM triethanolamine pH 8.0 buffer in 50 mM TRA pH 8.0 buffer | Homo sapiens | GTP | |
139.4 | - |
mutant enzyme R443S/G456A, in the absence of ADP, in 50 mM triethanolamine pH 8.0 buffer | Homo sapiens | GTP | |
166.8 | - |
GDH2, in the presence of 1 mM ADP, in 50 mM triethanolamine pH 8.0 buffer | Homo sapiens | GTP | |
622.4 | - |
mutant enzyme R443S/G456A, in the absence of ADP, in 50 mM triethanolamine pH 8.0 buffer | Homo sapiens | GTP |