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Literature summary for 1.4.1.3 extracted from

  • Choi, M.M.; Hwang, E.Y.; Kim, E.A.; Huh, J.W.; Cho, S.W.
    Identification of amino acid residues responsible for different GTP preferences of human glutamate dehydrogenase isozymes (2008), Biochem. Biophys. Res. Commun., 368, 742-747.
    View publication on PubMed
Search for more literature for 1.4.1.3

Cloned(Commentary)

Cloned (Comment) Organism
chimeric mutant enzymes expressed in Escherichia coli DE3 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
L415M/S443R/A456G triple mutant hGDH2(hGDH1390-465)hGDH2 (amino acid segment 390-465 of hGDH2 replaced by the corresponding hGDH1 segment) Homo sapiens
M415L/R443S/G456A triple mutant hGDH1(hGDH2390-465)hGDH1 (amino acid segment 390-465 of hGDH1 replaced by the corresponding hGDH2 segment) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
GTP
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.075
-
 NADH mutant enzyme L415M/S443R/A456G, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C Homo sapiens
0.08
-
 NADH mutant enzyme M415L/R443S/G456A, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C Homo sapiens
0.081
-
 NADH wild type isozyme GDH1, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C Homo sapiens
0.086
-
 NADH wild type isozyme GDH2, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C Homo sapiens
1.25
-
 2-oxoglutarate wild type isozyme GDH1, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C Homo sapiens
1.27
-
 2-oxoglutarate mutant enzyme L415M/S443R/A456G, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C Homo sapiens
1.29
-
 2-oxoglutarate mutant enzyme M415L/R443S/G456A, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C Homo sapiens
1.39
-
 2-oxoglutarate wild type isozyme GDH2, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2',5'-ADP Sepharose 4B column and FPLC Resource-Q column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD+
-
 Homo sapiens 2-oxoglutarate + NH3 + NADH + H+
-
 ?
L-glutamate + H2O + NADP+
-
 Homo sapiens 2-oxoglutarate + NH3 + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
GDH1 isozyme Homo sapiens
GDH2 isozyme Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
101
-
 NADH mutant enzyme M415L/R443S/G456A, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C Homo sapiens
104
-
 NADH wild type isozyme GDH1, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C Homo sapiens
118
-
 NADH mutant enzyme L415M/S443R/A456G, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C Homo sapiens
130
-
 NADH wild type isozyme GDH2, 50 mM triethanolamine pH 8.0, 100 mM ammonium acetate, 0.1 mM NADH, and 2 mM EDTA, pH 8.0 at 25°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD(P)+
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.001
-
 isozyme GDH1 Homo sapiens GTP
0.07
-
 isozyme GDH2 Homo sapiens GTP