KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADPH | pH 7.6, 60°C | Saccharolobus solfataricus | |
0.14 | - |
NADH | pH 7.6, 50°C | Saccharolobus solfataricus | |
1.4 | - |
2-oxoglutarate | pH 7.6, 60°C | Saccharolobus solfataricus | |
2.5 | - |
L-glutamate | pH 7.6, 60°C | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
6 * 45000, SDS-PAGE | Saccharolobus solfataricus |
270000 | - |
gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Saccharolobus solfataricus |
Storage Stability | Organism |
---|---|
4°C, 1 mg/ml protein concentration, glutamate dehydrogenase remains stable for at least one month | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH3 + NADH | specific activity in the presence of NADH is about 30% of that with NADPH | Saccharolobus solfataricus | L-glutamate + H2O + NAD+ | - |
r | |
2-oxoglutarate + NH3 + NADPH | - |
Saccharolobus solfataricus | L-glutamate + H2O + NADP+ | - |
r | |
L-glutamate + H2O + NADP+ | - |
Saccharolobus solfataricus | 2-oxoglutarate + NH3 + NADPH | - |
r | |
norvaline + H2O + NADP+ | activity is 20% of that observed in the presence of 2-oxoglutarate and L-glutamate | Saccharolobus solfataricus | 2-oxovalerate + NH3 + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 45000, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
NAD(P)-dependent glutamate dehydrogenase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
substrates: 2-oxoglutarate + NH3 + NADPH | Saccharolobus solfataricus |
70 | - |
substrates: L-glutamate + H2O + NADP+ | Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 75 | 45°C: about 35% of maximal activity, 75°C: about 20% of maximal activity, substrates: 2-oxoglutarate + NH3 + NADPH | Saccharolobus solfataricus |
60 | 75 | 60°C: about 60% of maximal activity, 75°C: about 30% of maximal activity, substrates: L-glutamate + H2O + NADP+ | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
48 h, protein concentration of 0.2 mg/ml, stable | Saccharolobus solfataricus |
60 | - |
48 h, protein concentration of 0.2 mg/ml, 20% loss of activity | Saccharolobus solfataricus |
80 | - |
half-life: 15 h | Saccharolobus solfataricus |
85 | - |
half-life: 2 h | Saccharolobus solfataricus |
90 | - |
half-life: 20 min | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
substrates: 2-oxoglutarate + NH3 + NADPH | Saccharolobus solfataricus |
10 | - |
substrates: L-glutamate + H2O + NADP+ | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Saccharolobus solfataricus | |
NADP+ | - |
Saccharolobus solfataricus | |
NADPH | - |
Saccharolobus solfataricus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharolobus solfataricus | isoelectric focusing. Microheterogeneity shows three active bands corresponding to pI of 5.7, 5.5 and 5.4. The major band shows an isoelectric point of 5.7 | - |
5.7 |