Cloned (Comment) | Organism |
---|---|
expression in Sf21 cells using a baculovirus expression system | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G456A | mutation renders the enzyme markedly resistant to GTP inhibition, mutation abolishes the cooperative behavior of the enzyme | Homo sapiens |
N498S | mutation does not render the enzyme resistant to GTP inhibition | Homo sapiens |
R470H | mutation does not render the enzyme resistant to GTP inhibition | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GTP | inhibition of wild-type enzyme and mutant enzymes r470H and N498S. No inhibition of mutant enzyme G456A. IC50 of wild-type enzyme is 0.00019 mM, IC50 of mutant enzyme G456A is 0.0028 mM, IC50 of mutant enzyme R470G is 0.00017 mM, IC50 of mutant enzyme N498S is 0.0002 mM | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00367 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NADH + NH3 | - |
Homo sapiens | L-glutamate + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GLUD1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00017 | - |
inhibition of wild-type enzyme and mutant enzymes r470H and N498S. No inhibition of mutant enzyme G456A. IC50 of mutant enzyme R470G is 0.00017 mM, IC50 of mutant enzyme N49 | Homo sapiens | GTP | |
0.00019 | - |
inhibition of wild-type enzyme and mutant enzymes r470H and N498S. No inhibition of mutant enzyme G456A. IC50 of wild-type enzyme is 0.00019 mM | Homo sapiens | GTP | |
0.0028 | - |
IC50 of mutant enzyme G456A is 0.0028 mM | Homo sapiens | GTP |