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Literature summary for 1.4.1.3 extracted from

  • Zaganas, I.; Plaitakis, A.
    Single amino acid substitution (G456A) in the vicinity of the GTP binding domain of human housekeeping glutamate dehydrogenase markedly attenuates GTP inhibition and abolishes the cooperative behavior of the enzyme (2002), J. Biol. Chem., 277, 26422-26428.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf21 cells using a baculovirus expression system Homo sapiens

Protein Variants

Protein Variants Comment Organism
G456A mutation renders the enzyme markedly resistant to GTP inhibition, mutation abolishes the cooperative behavior of the enzyme Homo sapiens
N498S mutation does not render the enzyme resistant to GTP inhibition Homo sapiens
R470H mutation does not render the enzyme resistant to GTP inhibition Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
GTP inhibition of wild-type enzyme and mutant enzymes r470H and N498S. No inhibition of mutant enzyme G456A. IC50 of wild-type enzyme is 0.00019 mM, IC50 of mutant enzyme G456A is 0.0028 mM, IC50 of mutant enzyme R470G is 0.00017 mM, IC50 of mutant enzyme N498S is 0.0002 mM Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P00367
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NADH + NH3
-
Homo sapiens L-glutamate + NAD+ + H2O
-
?

Synonyms

Synonyms Comment Organism
GLUD1
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADH
-
Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00017
-
inhibition of wild-type enzyme and mutant enzymes r470H and N498S. No inhibition of mutant enzyme G456A. IC50 of mutant enzyme R470G is 0.00017 mM, IC50 of mutant enzyme N49 Homo sapiens GTP
0.00019
-
inhibition of wild-type enzyme and mutant enzymes r470H and N498S. No inhibition of mutant enzyme G456A. IC50 of wild-type enzyme is 0.00019 mM Homo sapiens GTP
0.0028
-
IC50 of mutant enzyme G456A is 0.0028 mM Homo sapiens GTP