KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
NADPH | reductive amination, low-activity form of the enzyme | Bacteroides fragilis | |
0.013 | - |
NADPH | reductive amination, high-activity form of the enzyme | Bacteroides fragilis | |
0.013 | - |
NADP+ | oxidative deamination, low-activity form of the enzyme | Bacteroides fragilis | |
0.029 | - |
NADP+ | oxidative deamination, high-activity form of the enzyme | Bacteroides fragilis | |
0.13 | - |
NADH | reductive amination, low-activity form of the enzyme | Bacteroides fragilis | |
0.18 | - |
2-oxoglutarate | reductive amination, low-activity form of the enzyme | Bacteroides fragilis | |
0.2 | - |
2-oxoglutarate | reductive amination, high-activity form of the enzyme | Bacteroides fragilis | |
0.43 | - |
NAD+ | oxidative deamination, low-activity form of the enzyme | Bacteroides fragilis | |
0.52 | - |
NADH | reductive amination, high-activity form of the enzyme | Bacteroides fragilis | |
1.25 | - |
NH3 | reductive amination, high-activity form of the enzyme | Bacteroides fragilis | |
1.31 | - |
NH3 | reductive amination, low-activity form of the enzyme | Bacteroides fragilis | |
2.44 | - |
NAD+ | oxidative deamination, high-activity form of the enzyme | Bacteroides fragilis | |
3.65 | - |
L-glutamate | oxidative deamination, low-activity form of the enzyme | Bacteroides fragilis | |
3.83 | - |
L-glutamate | oxidative deamination, high-activity form of the enzyme | Bacteroides fragilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
6 * 49000, SDS-PAGE | Bacteroides fragilis |
290000 | - |
hig- and low-activity form, gel filtration | Bacteroides fragilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides fragilis | - |
high activity-form and low-activity form | - |
Purification (Comment) | Organism |
---|---|
- |
Bacteroides fragilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.9 | - |
low-activity form of the enzyme, NADP+ dependent deamination | Bacteroides fragilis |
32.3 | - |
high-activity form of the enzyme, NADP+-dependent deamination | Bacteroides fragilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH3 + NADH + H+ | - |
Bacteroides fragilis | L-glutamate + H2O + NAD+ | - |
r | |
2-oxoglutarate + NH3 + NADH + H+ | NH4Cl used in enzyme assay | Bacteroides fragilis | L-glutamate + H2O + NAD+ | - |
r | |
L-glutamate + H2O + NAD(P)+ | - |
Bacteroides fragilis | 2-oxoglutarate + NH3 + NAD(P)H | - |
r | |
L-glutamate + H2O + NAD+ | - |
Bacteroides fragilis | 2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 49000, SDS-PAGE | Bacteroides fragilis |