Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Bacteroides fragilis | |
D-glutamate | 10 mM, 57% inhibition of NADP+-linked activity, 30% inhibition of NADPH-linked activity | Bacteroides fragilis | |
glutamate | - |
Bacteroides fragilis | |
NaCl | 100 mM, 50% inhibition of NADH and NAD+ dependent reactions | Bacteroides fragilis | |
oxaloacetate | 5 mM, 20-25% inhibition of NADH- and NAD+-dependent activities | Bacteroides fragilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
NADPH | NADP-linked reductive amination | Bacteroides fragilis | |
0.019 | - |
NADP+ | NADP-linked oxidative deamination | Bacteroides fragilis | |
0.14 | - |
2-oxoglutarate | NADP-linked reductive amination | Bacteroides fragilis | |
0.2 | - |
NADH | NAD-linked reductive amination | Bacteroides fragilis | |
1.7 | - |
NH3 | NADP-linked reductive amination | Bacteroides fragilis | |
2.4 | - |
L-glutamate | NADP-linked oxidative deamination | Bacteroides fragilis | |
3 | - |
NAD+ | NAD-linked oxidative deamination | Bacteroides fragilis | |
4.9 | - |
NH3 | NAD-linked reductive amination | Bacteroides fragilis | |
5.1 | - |
NH3 | NADP-linked reductive amination | Bacteroides fragilis | |
7.1 | - |
2-oxoglutarate | NAD-linked reductive amination | Bacteroides fragilis | |
7.3 | - |
L-glutamate | NAD-linked oxidative deamination | Bacteroides fragilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
300000 | 350000 | polyacrylamide disc gel electrophoresis | Bacteroides fragilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides fragilis | - |
strictly anaerobic bacterium | - |
Purification (Comment) | Organism |
---|---|
- |
Bacteroides fragilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.4 | - |
- |
Bacteroides fragilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobutyrate + H2O + NADP+ | 3% of activity with L-glutamate | Bacteroides fragilis | 2-oxobutyrate + NH3 + NADPH | - |
? | |
2-oxoglutarate + NH3 + NADH + H+ | - |
Bacteroides fragilis | L-glutamate + H2O + NAD+ | - |
r | |
2-oxoglutarate + NH3 + NADH + H+ | NH4Cl used in enzyme assay | Bacteroides fragilis | L-glutamate + H2O + NAD+ | - |
r | |
L-glutamate + H2O + NAD(P)+ | - |
Bacteroides fragilis | 2-oxoglutarate + NH3 + NAD(P)H | - |
r | |
L-glutamate + H2O + NAD+ | - |
Bacteroides fragilis | 2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
valine + H2O + NADP+ | 3% of activity with L-glutamate | Bacteroides fragilis | 2-oxovalerate + NH3 + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | SDS-PAGE | Bacteroides fragilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
NADH + 2-oxoglutarate + NH3 | Bacteroides fragilis |
8 | - |
NADPH + 2-oxoglutarate + NH3 | Bacteroides fragilis |
9 | - |
NADH + NH3 + 2-oxoglutarate | Bacteroides fragilis |
9.5 | - |
glutamate + NADP+ | Bacteroides fragilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | amination activity is 5fold higher with NADPH than that with NADH | Bacteroides fragilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
70 | - |
NaCl | - |
Bacteroides fragilis | |
80 | - |
L-glutamate | reductive amination with NADPH, not with NADH is inhibited | Bacteroides fragilis |