Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 2fold activation | Glycine max | |
glutathione | 2fold activation | Glycine max |
General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes | Glycine max |
glutathione stabilizes | Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Glycine max | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
300000 | 350000 | isoenzymes GDH1, GDH2, disc gel electrophoresis | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
glutamate dehydrogenase 1 and 2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Glycine max | - |
seed | glutamate dehydrogenase 1 is found only in the developing seed | Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.2 | - |
glutamate dehydrogenase 2 | Glycine max |
1.8 | - |
glutamate dehydrogenase 1 | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD(P)+ | - |
Glycine max | 2-oxoglutarate + NH3 + NAD(P)H | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
NADPH | Glycine max |
8 | - |
- |
Glycine max |
8.3 | - |
2-oxoglutarate + NH3 + NAD(P)H | Glycine max |
8.7 | - |
NADP+ + glutamate | Glycine max |
8.9 | - |
NAD+ + glutamate | Glycine max |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.8 | NAD(P)H + 2-oxoglutarate + NH3, more than 50% of activity maximum at pH 7.5 and 8.8 | Glycine max |
8 | 9.2 | deamination, more than 50% of activity maximum at pH 8.0 and 9.2 | Glycine max |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Glycine max | |
NADH | reaction rate NADH/NADPH: 4.5 (GDH2) | Glycine max | |
NADH | reaction rate NADH/NADPH: 1.3 (GDH1) | Glycine max | |
NADP+ | - |
Glycine max | |
NADPH | glutamate dehydrogenase 2, reaction rate NADH/NADPH: 4.4 | Glycine max | |
NADPH | glutamate dehydrogenase 1, reaction rate NADH/NADPH: 1.3 | Glycine max |