Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-valine | substrate inhibition, noncompetitive | Streptomyces coelicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
2 * 41000, SDS-PAGE | Streptomyces coelicolor |
70000 | - |
gel filtration | Streptomyces coelicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
- |
- |
Streptomyces coelicolor A3(2) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces coelicolor |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-valine + H2O + NAD+ = 3-methyl-2-oxobutanoate + NH3 + NADH + H+ | sequential ordered mechanism with non-competitive inhibition by valine | Streptomyces coelicolor |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14 | - |
pH 10.4, 30°C, substrate L-valine | Streptomyces coelicolor |
19 | - |
pH 10.4, 30°C, substrate 2-aminobutanoate | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-aminobutanoate + H2O + NAD+ | 130% of the activity with L-valine | Streptomyces coelicolor | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-alanine + H2O + NAD+ | 3% of the activity with L-valine | Streptomyces coelicolor | pyruvate + NH3 + NADH + H+ | - |
r | |
L-isoleucine + H2O + NAD+ | 28% of the activity with L-valine | Streptomyces coelicolor | 3-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
L-leucine + H2O + NAD+ | 8% of the activity with L-valine | Streptomyces coelicolor | 2-oxoisohexanoate + NH3 + NADH + H+ | - |
r | |
L-valine + H2O + NAD+ | - |
Streptomyces coelicolor | 3-methyl-2-oxobutanoate + NH3 + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41000, SDS-PAGE | Streptomyces coelicolor |
Synonyms | Comment | Organism |
---|---|---|
VDH | - |
Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
reductive amination | Streptomyces coelicolor |
10.5 | - |
oxidative deamination | Streptomyces coelicolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor: NADP+ | Streptomyces coelicolor | |
NAD+ | - |
Streptomyces coelicolor | |
NADH | - |
Streptomyces coelicolor |
Organism | Comment | Expression |
---|---|---|
Streptomyces coelicolor | activity is repressed in the presence of glucose and NH4+ or glycerol and NH4+ or asparagine | down |
Streptomyces coelicolor | activity is induced in presence of D- and L-valine | up |