KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
L-leucine | pH 10.6, 30°C | Streptomyces fradiae | |
0.8 | - |
2-oxoisovalerate | pH 8.8, 30°C | Streptomyces fradiae | |
0.82 | - |
2-Oxoisohexanoate | pH 8.8, 30°C | Streptomyces fradiae | |
1 | - |
2-oxobutanoate | pH 8.8, 30°C | Streptomyces fradiae | |
1 | - |
L-valine | pH 10.6, 30°C | Streptomyces fradiae | |
1.18 | - |
L-isoleucine | pH 10.6, 30°C | Streptomyces fradiae | |
1.33 | - |
L-norvaline | pH 10.6, 30°C | Streptomyces fradiae | |
1.94 | - |
2-oxovalerate | pH 8.8, 30°C | Streptomyces fradiae | |
2.23 | - |
2-Oxohexanoate | pH 8.8, 30°C | Streptomyces fradiae | |
3.07 | - |
L-2-aminobutanoate | pH 10.6, 30°C | Streptomyces fradiae | |
3.33 | - |
3-methyl-2-oxopentanoate | pH 8.8, 30°C | Streptomyces fradiae | |
6.68 | - |
L-norleucine | pH 10.6, 30°C | Streptomyces fradiae | |
25 | - |
pyruvate | pH 8.8, 30°C | Streptomyces fradiae | |
28.2 | - |
L-alanine | pH 10.6, 30°C | Streptomyces fradiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
12 * 18000, SDS-PAGE | Streptomyces fradiae |
215000 | 218000 | gel filtration and equilibrium ultracentrifugation | Streptomyces fradiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces fradiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces fradiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + NH3 + NADH + H+ | 78% of the activity with 2-oxoisovalerate | Streptomyces fradiae | L-2-aminobutanoate + H2O + NAD+ | - |
r | |
2-oxohexanoate + NH3 + NADH + H+ | 43.8% of the activity with 2-oxoisovalerate | Streptomyces fradiae | L-norleucine + H2O + NAD+ | - |
r | |
2-oxoisohexanoate + NH3 + NADH + H+ | 20% of the activity with 2-oxoisovalerate | Streptomyces fradiae | L-leucine + H2O + NAD+ | - |
r | |
2-oxoisovalerate + NH3 + NADH + H+ | - |
Streptomyces fradiae | L-valine + H2O + NAD+ | - |
r | |
2-oxovalerate + H2O + NAD+ | 79.5% of the activity with 2-oxoisovalerate | Streptomyces fradiae | L-norvaline + NH3 + NADH + H+ | - |
r | |
3-methyl-2-oxopentanoate + NH3 + NADH + H+ | 23.2% of the activity with 2-oxoisovalerate | Streptomyces fradiae | L-isoleucine + H2O + NAD+ | - |
r | |
L-2-aminobutanoate + H2O + NAD+ | 68.9% of the activity with L-valine | Streptomyces fradiae | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-alanine + H2O + NAD+ | 14.7% of the activity with L-valine | Streptomyces fradiae | pyruvate + NH3 + NADH + H+ | - |
r | |
L-isoleucine + H2O + NAD+ | 29.4% of the activity with L-valine | Streptomyces fradiae | 3-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
L-leucine + H2O + NAD+ | 24.5% of the activity with L-valine | Streptomyces fradiae | 2-oxoisohexanoate + NH3 + NADH + H+ | - |
r | |
L-norleucine + H2O + NAD+ | 51.5% of the activity with L-valine | Streptomyces fradiae | 2-oxohexanoate + NH3 + NADH + H+ | - |
r | |
L-norvaline + H2O + NAD+ | 98% of the activity with L-valine | Streptomyces fradiae | 2-oxovalerate + NH3 + NADH + H+ | - |
r | |
L-valine + H2O + NAD+ | - |
Streptomyces fradiae | 3-methyl-2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
additional information | no substrates: D-valine, D-leucine, D-isoleucine, glycine, L-threonine, fialanine, L-serine, L-cysteine, L-methionine, L-glutamic acid, L-aspartic acid, L-asparagine, L-glutamine, L-lysine, L-phenylalanine, L-tyrosine, L-histidine and L-tryptophan as well as 2-oxoglutarate, oxalacetate, phenylpyruvate and glyoxalate | Streptomyces fradiae | ? | - |
? | |
pyruvate + NH3 + NADH + H+ | 31.6% of the activity with 2-oxoisovalerate | Streptomyces fradiae | L-alanine + H2O + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dodecamer | 12 * 18000, SDS-PAGE | Streptomyces fradiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
reductive amination | Streptomyces fradiae |
10.6 | - |
oxidative deamination | Streptomyces fradiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
1,N6-etheno-NAD+ | 64.4% of the activity with NAD+ | Streptomyces fradiae | |
3-acetylpyridine-NAD+ | 64.5% of the activity with NAD+ | Streptomyces fradiae | |
Alpha-NAD+ | 3.6% of the activity with NAD+ | Streptomyces fradiae | |
deamino-NAD+ | 93.3% of the activity with NAD+ | Streptomyces fradiae | |
additional information | no cofactors: deamido-NAD+, 3-pyridinealdehyde-NAD+, thionicotinamide-NAD+ | Streptomyces fradiae | |
NAD+ | - |
Streptomyces fradiae | |
NADH | - |
Streptomyces fradiae | |
NADP+ | 10.8% of the activity with NAD+ | Streptomyces fradiae | |
nicotinamide guanine dinucleotide | 92.9% of the activity with NAD+ | Streptomyces fradiae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces fradiae | isoelectric focusing | - |
4.7 |