Cloned (Comment) | Organism |
---|---|
AspDH gene cluster, gene AspDH expression in Escherichia coli strain BL21(DE3), quantitative real-time PCR expression analysis | Cupriavidus necator |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an AspDH knockout strain, growth phenotype, compared to the wild-type | Cupriavidus necator |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
NADH | pH 8.2, 37°C | Cupriavidus necator | |
0.11 | - |
NAD+ | pH 10.2, 37°C | Cupriavidus necator | |
0.19 | - |
L-aspartate | pH 10.2, 37°C, with NAD+ | Cupriavidus necator | |
0.32 | - |
NADP+ | pH 10.2, 37°C | Cupriavidus necator | |
1.2 | - |
oxaloacetate | pH 8.2, 37°C, with NADH | Cupriavidus necator | |
4.3 | - |
L-aspartate | pH 10.2, 37°C, with NADP+ | Cupriavidus necator | |
167 | - |
NH3 | pH 8.2, 37°C, with NADH | Cupriavidus necator |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000, SDS-PAGE | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + H2O + NAD(P)+ | Cupriavidus necator | - |
oxaloacetate + NH3 + NAD(P)H + H+ | - |
r | |
L-aspartate + H2O + NAD(P)+ | Cupriavidus necator JMP 134-1 | - |
oxaloacetate + NH3 + NAD(P)H + H+ | - |
r | |
additional information | Cupriavidus necator | the wild-type strain synthesizes 3-hydroxy-polybutyrate from fructose or L-Asp, while the enzyme knockout mutant strain does not | ? | - |
? | |
additional information | Cupriavidus necator JMP 134-1 | the wild-type strain synthesizes 3-hydroxy-polybutyrate from fructose or L-Asp, while the enzyme knockout mutant strain does not | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | Q46VA0 | gene B3576 | - |
Cupriavidus necator JMP 134-1 | Q46VA0 | gene B3576 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
137 | - |
37°C, pH 10.2 | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + H2O + NAD(P)+ | - |
Cupriavidus necator | oxaloacetate + NH3 + NAD(P)H + H+ | - |
r | |
L-aspartate + H2O + NAD(P)+ | - |
Cupriavidus necator JMP 134-1 | oxaloacetate + NH3 + NAD(P)H + H+ | - |
r | |
L-aspartate + H2O + NAD+ | - |
Cupriavidus necator | oxaloacetate + NH3 + NADH + H+ | - |
r | |
L-aspartate + H2O + NAD+ | - |
Cupriavidus necator JMP 134-1 | oxaloacetate + NH3 + NADH + H+ | - |
r | |
L-aspartate + H2O + NADP+ | - |
Cupriavidus necator | oxaloacetate + NH3 + NADPH + H+ | - |
r | |
L-aspartate + H2O + NADP+ | - |
Cupriavidus necator JMP 134-1 | oxaloacetate + NH3 + NADPH + H+ | - |
r | |
additional information | the wild-type strain synthesizes 3-hydroxy-polybutyrate from fructose or L-Asp, while the enzyme knockout mutant strain does not | Cupriavidus necator | ? | - |
? | |
additional information | the wild-type strain synthesizes 3-hydroxy-polybutyrate from fructose or L-Asp, while the enzyme knockout mutant strain does not | Cupriavidus necator JMP 134-1 | ? | - |
? | |
oxaloacetate + NH3 + NADH + H+ | - |
Cupriavidus necator | L-aspartate + H2O + NAD+ | - |
r | |
oxaloacetate + NH3 + NADH + H+ | - |
Cupriavidus necator JMP 134-1 | L-aspartate + H2O + NAD+ | - |
r | |
oxaloacetate + NH3 + NADPH + H+ | - |
Cupriavidus necator | L-aspartate + H2O + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 28000, SDS-PAGE | Cupriavidus necator |
Synonyms | Comment | Organism |
---|---|---|
L-aspartate dehydrogenase | - |
Cupriavidus necator |
L-aspDH | - |
Cupriavidus necator |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Cupriavidus necator |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | activity range, profile overview | Cupriavidus necator |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
20 min, fully stable up to | Cupriavidus necator |
49 | - |
20 min, Tm of purified enzyme | Cupriavidus necator |
55 | - |
20 min, inactivation | Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
amination | Cupriavidus necator |
10.2 | - |
deamination | Cupriavidus necator |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.8 | 7.2 | stable | Cupriavidus necator |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | L-AspDH can utilize both NAD+ and NADP+ as a coenzyme, albeit at different efficiencies, approximately 8fold higher Km value for NADP+ over NAD+ | Cupriavidus necator | |
NAD+ | - |
Cupriavidus necator | |
NADH | - |
Cupriavidus necator | |
NADP+ | - |
Cupriavidus necator | |
NADPH | - |
Cupriavidus necator |
General Information | Comment | Organism |
---|---|---|
physiological function | the wild-type strain synthesizes 3-hydroxy-polybutyrate from fructose or L-Asp, while the enzyme knockout mutant strain does not. The AspDH cluster might be involved in the biosynthesis of poly-3-hydroxyalkanoates | Cupriavidus necator |