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Literature summary for 1.4.1.21 extracted from

  • Yoneda, K.; Sakuraba, H.; Tsuge, H.; Katunuma, N.; Ohshima, T.
    Crystal structure of archaeal highly thermostable L-aspartate dehydrogenase/NAD/citrate ternary complex (2007), FEBS J., 274, 4315-4325.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Thermotoga maritima
expressed in Escherichia coli Archaeoglobus fulgidus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion method with 100 mM phosphate-citrate buffer pH 4.2 (60.5 mM, Na2HPO4, 39.5 mM citric acid), 5% (v/v) polyethylene glycol 3000 (PEG 3000), 10% (v/v) glycerol and 22% (v/v) 1,2-propanediol Thermotoga maritima
sitting drop vapour diffusion method with 100 mM phosphate-citrate buffer pH 4.2 (60.5 mM, Na2HPO4, 39.5 mM citric acid), 5% (v/v) polyethylene glycol 3000 (PEG 3000), 10% (v/v) glycerol and 22% (v/v) 1,2-propanediol Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O28440
-
-
Thermotoga maritima Q9X1X6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermotoga maritima
-
Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + NAD+
-
Thermotoga maritima oxaloacetate + NH4+ + NADH
-
?
L-aspartate + NAD+
-
Archaeoglobus fulgidus oxaloacetate + NH4+ + NADH
-
?

Subunits

Subunits Comment Organism
dimer x-ray crystallography Thermotoga maritima
tetramer x-ray crystallography Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
L-aspartate dehydrogenase
-
Thermotoga maritima
L-aspartate dehydrogenase
-
Archaeoglobus fulgidus
L-aspDH
-
Thermotoga maritima
L-aspDH
-
Archaeoglobus fulgidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
the half life at 100°C is 10 min Archaeoglobus fulgidus
100
-
the half life at 100°C is 10.7 min Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Thermotoga maritima
NAD+
-
Archaeoglobus fulgidus