Literature summary for 1.4.1.20 extracted from

Zhao, C.; Wang, L.; Li, D.; Li, J.; Ge, Y.; Liang, T.; Zhu, Y.; Yu, R.
High-cell-density fermentation of Escherichia coli for expression of a recombinant phenylalanine dehydrogenase mutant and its purification (2021), J. Chem. Technol. Biotechnol., 96, 199-206 .

No PubMed abstract available

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Application

Application	Comment	Organism
synthesis	expression of mutant R272M/E331Q/E196N in Escherichia coli via a fed-batch fermentation strategy. After 24 h of cultivation, the specific activity of crude extracts is 0.49 U/mg. The specific activity, recovery, yield and purification fold of PheDH are 3.21 U/mg, 104.19%, 84.53% and 6.55%, respectively	Thermoactinomyces intermedius

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Thermoactinomyces intermedius

Protein Variants

Protein Variants	Comment	Organism
R272M/E331Q/E196N	mutant with improved capability of catalyzing 2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid to the corresponding non-natural amino acid (S)-3-hydroxyadamantylglycine which is the key intermediate of saxagliptin	Thermoactinomyces intermedius

Organism

Organism	UniProt	Comment	Textmining
Thermoactinomyces intermedius	P22823	-	-

Subunits

Subunits	Comment	Organism
?	x * 40000, SDS-PAGE	Thermoactinomyces intermedius

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Release 2023.1 (January 2023)