Cloned (Comment) | Organism |
---|---|
espressed as recombinant protein in Escherichia coli strain BL21 (DE3) | Bacillus badius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus badius | - |
- |
- |
Purification (Comment) | Organism |
---|---|
best purification results of recombinant PheDH are achieved in an polymer/salt aqueous two-phase systems (ATPS) with 9% of phenazine ethosulfate with a molecular mass of 6000 Da (PEG-6000) and 16% of K2HPO4 | Bacillus badius |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
553.6 | - |
after gel filtration protocol | Bacillus badius |
4231 | - |
after polymer/salt aqueous two-phase systems (ATPS) protocol | Bacillus badius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + H2O + NAD+ | - |
Bacillus badius | phenylpyruvate + NH3 + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PheDH | - |
Bacillus badius |
phenylalanine dehydrogenase | - |
Bacillus badius |
recombinant PheDH | - |
Bacillus badius |
recombinant phenylalanine dehydrogenase | - |
Bacillus badius |