Cloned (Comment) | Organism |
---|---|
the modified enzyme (contains two amino acid changes at the C-terminus and a 12 amino acid extension of the C-terminus) is expressed in Pichia pastoris strain SMD1168 or Escherichia coli strain SC 16496 | Thermoactinomyces intermedius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
subunit of modified PDH (contains two amino acid changes at the C-terminus and a 12 amino acid extension of the C-terminus), SDS-PAGE | Thermoactinomyces intermedius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoactinomyces intermedius | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification by Superdex 200 gel filtration | Thermoactinomyces intermedius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid + NH3 + NADH | modified PDH which contains two amino acid changes at the C-terminus and a 12 amino acid extension of the C-terminus is more effective with keto acid 2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid and less effective with phenylpyruvate than the wild type PDH | Thermoactinomyces intermedius | (S)-3-hydroxyadamantylglycine + H2O + NAD+ | - |
? | |
phenylpyruvate + NH3 + NADH | - |
Thermoactinomyces intermedius | L-Phe + H2O + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PDH | - |
Thermoactinomyces intermedius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
wild type PDH retains most of its activity after 1 h at 60°C whereas modified PDH which contains two amino acid changes at the C-terminus and a 12 amino acid extension of the C-terminus loses all activity after 1h at 60°C | Thermoactinomyces intermedius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | 9 | modified PDH which contains two amino acid changes at the C-terminus and a 12 amino acid extension of the C-terminus | Thermoactinomyces intermedius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermoactinomyces intermedius |