Literature summary for 1.4.1.20 extracted from

Villalonga, R.; Tachibana, S.; Perez, Y.; Asano, Y.
Increased conformational and thermal stability properties for phenylalanine dehydrogenase by chemical glycosidation with end-group activated dextran (2005), Biotechnol. Lett., 27, 1311-1317.

Search for more literature for 1.4.1.20

General Stability

General Stability	Organism
the enzyme chemically glycosylated with an end-group aminated dextran shows improved thermal and conformational stability	Bacillus badius

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.164	-	L-Phe	25°C, pH 10.4, dextran-modified enzyme	Bacillus badius
0.215	-	L-Phe	25°C, pH 10.4, native enzyme	Bacillus badius

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
325600	-	native enzyme, gel filtration	Bacillus badius
342000	-	dextran-modified enzyme, gel filtration	Bacillus badius

Organism

Organism	UniProt	Comment	Textmining
Bacillus badius	-	recombinantly expressed in Escherichia coli	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Phe + H2O + NAD+	-	Bacillus badius	phenylpyruvate + NH3 + NADH	-	?

Synonyms

Synonyms	Comment	Organism
PheDH	-	Bacillus badius

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	native enzyme	Bacillus badius
50	-	dextran-modified enzyme	Bacillus badius

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
25	60	20°C: about 60% of maximal activity, 55°C: about 85% of maximal activity, 60°C: about 35% of maximal activity	Bacillus badius
25	55	20°C: about 60% of maximal activity, 55°C, about 65% of maximal activity, native enzyme	Bacillus badius

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	-	10 min, native enzyme is stable up to	Bacillus badius
54	-	T50-value of native enzyme	Bacillus badius
55	-	10 min, dextran-modified enzyme is stable up to	Bacillus badius
62	-	T50-value of the dextran-modified enzyme	Bacillus badius

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
714	-	L-Phe	25°C, pH 10.4, dextran-modified enzyme	Bacillus badius
816	-	L-Phe	25°C, pH 10.4, native enzyme	Bacillus badius

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Release 2023.1 (January 2023)