Application | Comment | Organism |
---|---|---|
analysis | the chimeric enzyme has a specific activity of 6% of that of the parental phenylalanine dehydrogenase | Thermoactinomyces intermedius |
Cloned (Comment) | Organism |
---|---|
chimeric enzyme consisting of the N-terminal domain of Thermoactinomyces intermedius phenylalanine dehydrogenase, containing the substrate-binding region and the C-terminal domain of leucine dehydrogenase from Bacillus stearothermophilus containing the NAD+-binding region, expression in Escherichia coli | Thermoactinomyces intermedius |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a chimeric enzyme consisting of the N-terminal domain of Thermoactinomyces intermedius phenylalanine dehydrogenase, containing the substrate-binding region and the C-terminal domain of leucine dehydrogenase from Bacillus stearothermophilus containing the NAD+-binding region. The chimeric enzyme has a specific activity of 6% of that of the parental phenylalanine dehydrogenase and shows a broad substrate specificity in the oxidative deamination, like phenylalanine dehydrogenase. However, it acts much more effectively than phenylalanine dehydrogenase on Ile and Val | Thermoactinomyces intermedius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
L-Leu | chimeric enzyme | Thermoactinomyces intermedius | |
0.11 | - |
L-Phe | - |
Thermoactinomyces intermedius | |
0.12 | - |
L-Phe | chimeric enzyme | Thermoactinomyces intermedius | |
0.17 | - |
NAD+ | - |
Thermoactinomyces intermedius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
chimeric enzyme consisting of the N-terminal domain of Thermoactinomyces intermedius phenylalanine dehydrogenase, containing the substrate-binding region and the C-terminal domain of leucine dehydrogenase from Bacillus stearothermophilus containing the NAD+-binding region, gel filtration | Thermoactinomyces intermedius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoactinomyces intermedius | - |
- |
- |
Purification (Comment) | Organism |
---|---|
construction of a chimeric enzyme consisting of the N-terminal domain of Thermoactinomyces intermedius phenylalanine dehydrogenase, containing the substrate-binding region and the C-terminal domain of leucine dehydrogenase from Bacillus stearothermophilus containing the NAD+-binding region | Thermoactinomyces intermedius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-4-methylpentanoate + NH3 + NADH | 16% of the activity with phenylpyruvate | Thermoactinomyces intermedius | L-Ile + NAD+ + H2O | - |
? | |
2-oxo-4-methylthiobutanoate + NH3 + NADH | 55% of the activity with phenylpyruvate | Thermoactinomyces intermedius | L-Met + NAD+ + H2O | - |
? | |
2-oxobutanoate + NH3 + NADH | 5.5% of the activity with phenylpyruvate | Thermoactinomyces intermedius | 2-aminobutanoate + NAD+ + H2O | - |
? | |
2-oxohexanoate + NH3 + NADH | 130% of the activity with phenylpyruvate | Thermoactinomyces intermedius | 2-aminohexanoate + H2O + NAD+ | - |
? | |
2-oxoisohexanoate + NH3 + NADH | 47% of the activity with phenylpyruvate | Thermoactinomyces intermedius | L-Leu + NAD+ + H2O | - |
? | |
2-oxopentanoate + NH3 + NADH | 37% of the activity with phenylpyruvate | Thermoactinomyces intermedius | L-norvaline + NAD+ + H2O | - |
? | |
4-hydroxyphenylpyruvate + NH3 + NADH | 80% of the activity with phenylpyruvate | Thermoactinomyces intermedius | L-Tyr + NAD+ + H2O | - |
? | |
alloisoleucine + H2O + NAD+ | 26% of the activity with L-Phe | Thermoactinomyces intermedius | 3-methyl-2-oxopentanoate + NADH + NH3 | - |
? | |
L-Met + H2O + NAD+ | 2.2% of the activity with L-Phe | Thermoactinomyces intermedius | 2-oxo-4-methylthiobutanoate + NH3 + NADH | - |
? | |
L-norleucine + H2O + NAD+ | 30% of the activity with L-Phe | Thermoactinomyces intermedius | 2-oxohexanoic acid + NADH + NH3 | - |
? | |
L-norvaline + H2O + NAD+ | 28% of the activity with L-Phe | Thermoactinomyces intermedius | 2-oxopentanoate + NH3 + NADH | - |
? | |
L-Phe + H2O + NAD+ | - |
Thermoactinomyces intermedius | phenylpyruvate + NH3 + NADH | - |
r | |
L-Tyr + H2O + NAD+ | 40% of the activity with L-Phe | Thermoactinomyces intermedius | 4-hydroxyphenylpyruvate + NH3 + NADH | - |
? | |
m-fluoro-DL-phenylalanine + H2O + NAD+ | as effective as L-Phe | Thermoactinomyces intermedius | 3-(3-fluorophenyl)-2-oxopropionate + NADH + NH3 | - |
? | |
additional information | specific activity of the chimeric enzyme is 6% of that of the parental phenylalanine dehydrogenase and shows a broad substrate specificity in the oxidative deamination, like phenylalanine dehydrogenase. However, it acts much more effectively than phenylalanine dehydrogenase on Ile and Val. The parent enzyme and the chimeric enzyme belong to the pro-S specific dehydrogenase | Thermoactinomyces intermedius | ? | - |
? | |
o-fluoro-DL-phenylalanine + H2O + NAD+ | 65% of the activity with L-Phe | Thermoactinomyces intermedius | 3-(2-fluorophenyl)-2-oxopropionate + NADH + NH3 | - |
? | |
p-amino-L-phenylalanine + H2O + NAD+ | - |
Thermoactinomyces intermedius | ? | - |
? | |
p-fluoro-DL-phenylalanine + H2O + NAD+ | 118% of the activity with L-Phe | Thermoactinomyces intermedius | (4-fluorophenyl)-2-oxopropionate + NADH + NH3 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
54 | - |
60 min, pH 7.0-9.5, chimeric enzyme is stable | Thermoactinomyces intermedius |
58 | - |
pH 7.0-9.5, 60 min, chimeric enzyme, loss of activity above | Thermoactinomyces intermedius |
65 | 70 | 60 min, stable | Thermoactinomyces intermedius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.6 | - |
reductive amination of phenylpyruvate, chimeric enzyme | Thermoactinomyces intermedius |
10.7 | 11 | oxidative deamination of L-Phe, chimeric enzyme | Thermoactinomyces intermedius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermoactinomyces intermedius | |
NADH | - |
Thermoactinomyces intermedius |