Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.1.20 extracted from

  • Asano, Y.; Nakazawa, A.; Endo, K.; Hibino, Y.; Ohmori, M.; Numao, N.; Kondo, K.
    Phenylalanine dehydrogenase of Bacillus badius. Purification, characterization and gene cloning [published erratum appears in Eur J Biochem 1988 Jan 1;170(3):667] (1987), Eur. J. Biochem., 168, 153-159.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed at high levels in Escherichia coli Bacillus badius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
360000
-
gel filtration Bacillus badius

Organism

Organism UniProt Comment Textmining
Bacillus badius
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus badius

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
67.8
-
-
Bacillus badius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-4-methylpentanoate + NH3 + NADH 13% of the activity with phenylpyruvate Bacillus badius L-Ile + NAD+ + H2O
-
?
2-oxo-4-methylthiobutanoate + NH3 + NADH 16% of the activity with phenylpyruvate Bacillus badius L-Met + NAD+ + H2O
-
?
2-oxohexanoate + NH3 + NADH 31% of the activity with phenylpyruvate Bacillus badius 2-aminohexanoate + H2O + NAD+
-
?
2-oxopentanoate + NH3 + NADH 12% of the activity with phenylpyruvate Bacillus badius L-norvaline + NAD+ + H2O
-
?
4-hydroxyphenylpyruvate + NH3 + NADH 53% of the activity with phenylpyruvate Bacillus badius L-Tyr + NAD+ + H2O
-
?
alloisoleucine + H2O + NAD+ 4.3% of the activity with L-Phe Bacillus badius 3-methyl-2-oxopentanoate + NADH + NH3
-
?
L-2-amino-n-butyric acid + H2O + NAD+ 1.0% of the activity with L-Phe Bacillus badius 2-oxobutanoate + NADH + NH3
-
?
L-2-aminohexanoic acid + H2O + NAD+ 19% of the activity with L-Phe Bacillus badius 2-oxohexanoate + NADH + NH3
-
?
L-ethionine + H2O + NAD+ 7.0% of the activity with L-Phe Bacillus badius 4-ethylthio-2-oxobutanoate + NH3 + NADH
-
?
L-Leu + H2O + NAD+ 3.0% of the activity with L-Phe Bacillus badius 3-methyl-2-oxopentanoic acid + NH3 + NADH
-
?
L-Met + H2O + NAD+ 8.0% of the activity with L-Phe Bacillus badius 2-oxo-4-methylthiobutanoate + NH3 + NADH
-
?
L-norleucine + H2O + NAD+ 19% of the activity with L-Phe Bacillus badius 2-oxohexanoic acid + NADH + NH3
-
?
L-norvaline + H2O + NAD+ 5.0% of the activity with L-Phe Bacillus badius 2-oxopentanoate + NH3 + NADH
-
?
L-Phe + H2O + NAD+
-
Bacillus badius phenylpyruvate + NH3 + NADH
-
r
L-phenylalanine methyl ester + H2O + NAD+ 38% of the activity with L-Phe Bacillus badius phenylpyruvic acid methyl ester + NH3 + NADH
-
?
L-phenylalaninol + H2O + NAD+ 9.4% of the activity with L-Phe Bacillus badius 1-hydroxyacetone + NADH + NH3
-
?
L-Trp + H2O + NAD+ 4% of the activity with L-Phe Bacillus badius indole-3-pyruvate + NH3 + NADH
-
?
L-Tyr + H2O + NAD+ 9.0% of the activity with L-Phe Bacillus badius 4-hydroxyphenylpyruvate + NH3 + NADH
-
?
L-Val + H2O + NAD+ 4.0% of the activity with L-Phe Bacillus badius 3-methyl-2-oxobutanoate + NH3 + NADH
-
?
m-fluoro-DL-phenylalanine + H2O + NAD+ 11% of the activity with L-Phe Bacillus badius 3-(3-fluorophenyl)-2-oxopropionate + NADH + NH3
-
?
o-fluoro-DL-phenylalanine + H2O + NAD+ 2% of the activity with L-Phe Bacillus badius 3-(2-fluorophenyl)-2-oxopropionate + NADH + NH3
-
?
p-fluoro-DL-phenylalanine + H2O + NAD+ 34% of the activity with L-Phe Bacillus badius (4-fluorophenyl)-2-oxopropionate + NADH + NH3
-
?
phenylalaninamide + H2O + NAD+ 9.0% of the activity with L-Phe Bacillus badius 2-oxo-3-phenylpropionamide + NADH + NH3
-
?
phenylalanine hydroxamate + H2O + NAD+ 1.0% of the activity with L-Phe Bacillus badius 2-oxo-3-phenylpropionic acid hydroxamate + NADH + NH3
-
?

Subunits

Subunits Comment Organism
octamer 8 * 41000-42000, SDS-PAGE Bacillus badius

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bacillus badius
NADH
-
Bacillus badius