Cloned (Comment) | Organism |
---|---|
expressed at high levels in Escherichia coli | Bacillus badius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
360000 | - |
gel filtration | Bacillus badius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus badius | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus badius |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
67.8 | - |
- |
Bacillus badius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-4-methylpentanoate + NH3 + NADH | 13% of the activity with phenylpyruvate | Bacillus badius | L-Ile + NAD+ + H2O | - |
? | |
2-oxo-4-methylthiobutanoate + NH3 + NADH | 16% of the activity with phenylpyruvate | Bacillus badius | L-Met + NAD+ + H2O | - |
? | |
2-oxohexanoate + NH3 + NADH | 31% of the activity with phenylpyruvate | Bacillus badius | 2-aminohexanoate + H2O + NAD+ | - |
? | |
2-oxopentanoate + NH3 + NADH | 12% of the activity with phenylpyruvate | Bacillus badius | L-norvaline + NAD+ + H2O | - |
? | |
4-hydroxyphenylpyruvate + NH3 + NADH | 53% of the activity with phenylpyruvate | Bacillus badius | L-Tyr + NAD+ + H2O | - |
? | |
alloisoleucine + H2O + NAD+ | 4.3% of the activity with L-Phe | Bacillus badius | 3-methyl-2-oxopentanoate + NADH + NH3 | - |
? | |
L-2-amino-n-butyric acid + H2O + NAD+ | 1.0% of the activity with L-Phe | Bacillus badius | 2-oxobutanoate + NADH + NH3 | - |
? | |
L-2-aminohexanoic acid + H2O + NAD+ | 19% of the activity with L-Phe | Bacillus badius | 2-oxohexanoate + NADH + NH3 | - |
? | |
L-ethionine + H2O + NAD+ | 7.0% of the activity with L-Phe | Bacillus badius | 4-ethylthio-2-oxobutanoate + NH3 + NADH | - |
? | |
L-Leu + H2O + NAD+ | 3.0% of the activity with L-Phe | Bacillus badius | 3-methyl-2-oxopentanoic acid + NH3 + NADH | - |
? | |
L-Met + H2O + NAD+ | 8.0% of the activity with L-Phe | Bacillus badius | 2-oxo-4-methylthiobutanoate + NH3 + NADH | - |
? | |
L-norleucine + H2O + NAD+ | 19% of the activity with L-Phe | Bacillus badius | 2-oxohexanoic acid + NADH + NH3 | - |
? | |
L-norvaline + H2O + NAD+ | 5.0% of the activity with L-Phe | Bacillus badius | 2-oxopentanoate + NH3 + NADH | - |
? | |
L-Phe + H2O + NAD+ | - |
Bacillus badius | phenylpyruvate + NH3 + NADH | - |
r | |
L-phenylalanine methyl ester + H2O + NAD+ | 38% of the activity with L-Phe | Bacillus badius | phenylpyruvic acid methyl ester + NH3 + NADH | - |
? | |
L-phenylalaninol + H2O + NAD+ | 9.4% of the activity with L-Phe | Bacillus badius | 1-hydroxyacetone + NADH + NH3 | - |
? | |
L-Trp + H2O + NAD+ | 4% of the activity with L-Phe | Bacillus badius | indole-3-pyruvate + NH3 + NADH | - |
? | |
L-Tyr + H2O + NAD+ | 9.0% of the activity with L-Phe | Bacillus badius | 4-hydroxyphenylpyruvate + NH3 + NADH | - |
? | |
L-Val + H2O + NAD+ | 4.0% of the activity with L-Phe | Bacillus badius | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? | |
m-fluoro-DL-phenylalanine + H2O + NAD+ | 11% of the activity with L-Phe | Bacillus badius | 3-(3-fluorophenyl)-2-oxopropionate + NADH + NH3 | - |
? | |
o-fluoro-DL-phenylalanine + H2O + NAD+ | 2% of the activity with L-Phe | Bacillus badius | 3-(2-fluorophenyl)-2-oxopropionate + NADH + NH3 | - |
? | |
p-fluoro-DL-phenylalanine + H2O + NAD+ | 34% of the activity with L-Phe | Bacillus badius | (4-fluorophenyl)-2-oxopropionate + NADH + NH3 | - |
? | |
phenylalaninamide + H2O + NAD+ | 9.0% of the activity with L-Phe | Bacillus badius | 2-oxo-3-phenylpropionamide + NADH + NH3 | - |
? | |
phenylalanine hydroxamate + H2O + NAD+ | 1.0% of the activity with L-Phe | Bacillus badius | 2-oxo-3-phenylpropionic acid hydroxamate + NADH + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 41000-42000, SDS-PAGE | Bacillus badius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus badius | |
NADH | - |
Bacillus badius |