Literature summary for 1.4.1.20 extracted from

Asano, Y.; Endo, K.; Nakazawa, A.; Hibino, Y.; Okazaki, N.; Ohmori, M.; Numao, N.; Kondo, K.
Bacillus phenylalanine dehydrogenase produced in Escherichia coli - Its purification and application to L-phenylalanine synthesis (1987), Agric. Biol. Chem., 51, 2621-2623.

No PubMed abstract available

Search for more literature for 1.4.1.20

Application

Application	Comment	Organism
synthesis	formation of L-Phe from phenylpyruvate	Pediococcus acidilactici

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39000	-	8 * 39000, SDS-PAGE	Lysinibacillus sphaericus
310000	340000	gel filtration	Lysinibacillus sphaericus

Organism

Organism	UniProt	Comment	Textmining
Lysinibacillus sphaericus	-	recombinant enzyme, expressed in Escherichia coli	-
Pediococcus acidilactici	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Phe + H2O + NAD+	-	Lysinibacillus sphaericus	phenylpyruvate + NH3 + NADH	-	r
L-Phe + H2O + NAD+	-	Pediococcus acidilactici	phenylpyruvate + NH3 + NADH	-	r

Subunits

Subunits	Comment	Organism
octamer	8 * 39000, SDS-PAGE	Lysinibacillus sphaericus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Lysinibacillus sphaericus
NAD+	-	Pediococcus acidilactici
NADH	-	Lysinibacillus sphaericus
NADH	-	Pediococcus acidilactici

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)